Detailed information of TA system TA175102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1200268..1200745
• Replicon: chromosome
• Accession: NZ_CP062413
• Organism: Staphylococcus aureus strain NAS_AN_176

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF724_RS06075
• Protein ID: WP_000052076.1
• Coordinates: 1200557..1200745 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF724_RS06070
• Protein ID: WP_001059690.1
• Coordinates: 1200268..1200528 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF724_RS06035 (1195661)	1195661..1196377	-	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-
IF724_RS06040 (1196525)	1196525..1196947	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
IF724_RS06045 (1197346)	1197346..1197840	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF724_RS06050 (1197995)	1197995..1198612	+	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
IF724_RS06055 (1198685)	1198685..1199266	-	582	WP_001140693.1	histidine phosphatase family protein	-
IF724_RS06060 (1199491)	1199491..1199727	+	237	WP_000349581.1	hypothetical protein	-
IF724_RS06065 (1199724)	1199724..1199927	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF724_RS06070 (1200268)	1200268..1200528	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF724_RS06075 (1200557)	1200557..1200745	+	189	WP_000052076.1	hypothetical protein	Toxin
IF724_RS06080 (1200933)	1200933..1201502	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
IF724_RS06085 (1201590)	1201590..1201874	+	285	WP_001004399.1	hypothetical protein	-
IF724_RS06090 (1201936)	1201936..1202154	+	219	WP_000005886.1	hypothetical protein	-
IF724_RS06095 (1202646)	1202646..1202846	-	201	WP_001059082.1	cold-shock protein	-
IF724_RS06100 (1203203)	1203203..1203889	-	687	WP_000141557.1	thermonuclease family protein	-
IF724_RS06105 (1204230)	1204230..1204751	-	522	WP_049311898.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp	1196525..1230463	33938

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T175102 WP_000052076.1 NZ_CP062413:1200557-1200745 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T175102 NZ_CP081768:c43107-42802 [Escherichia coli]
ATGCAATTCATCATCTACGAATATAAGCGGGTAAGCCATTACAAAATGTTTGTCGATGTGCAAAGCGATATAGTTGATAC
ACCAGGCCGTCGTATGGTTATACCGCTGATTGAATCCCATCACTTGTCTGAAATCGTAAACAGCTCCCTGTTCCCGGTAG
TCAGAATTAACGATGAAGACTACCGGGTAATGACTACCGAGCTATCAAGCGTGTCTGCGGCGGTTACTGGTGAAGCTATT
GCCGATGTCAGCTCTGATGCAGATGCGATAAAAAATGCTATCAACCTTATGTTCTGGGGAATTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT175102 WP_001059690.1 NZ_CP062413:1200268-1200528 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT175102 NZ_CP081768:c43327-43109 [Escherichia coli]
ATGAAACACAGAGTCAGCGTTACAGTAGACAGAGAAAATTATCAGGTTCTGAGTTCTGCCGGAATCAACATTTCAGGGCT
GGTCAATGAGGTCATAGGCAAAGAAGCACGGAAGATAAAAGCCGAGCAATGGAAAGCGGACAACCGCGCAGGTATGGAGG
AAGTCGCAAACTTCATCGCGCAGCATGGCTCCTTTGCCGAAGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56