Detailed information of TA system TA175081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1613079..1613608
• Replicon: chromosome
• Accession: NZ_CP062412
• Organism: Staphylococcus aureus strain NAS_AN_181

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX2
• Locus tag: IF725_RS08005
• Protein ID: WP_001103933.1
• Coordinates: 1613079..1613396 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: A0A2X2M557
• Locus tag: IF725_RS08010
• Protein ID: WP_001058488.1
• Coordinates: 1613399..1613608 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF725_RS07980 (1608194)	1608194..1608535	-	342	WP_001190614.1	hypothetical protein	-
IF725_RS07985 (1609071)	1609071..1609712	-	642	WP_064140196.1	pathogenicity island protein	-
IF725_RS07990 (1609709)	1609709..1610089	-	381	WP_000356927.1	hypothetical protein	-
IF725_RS07995 (1610420)	1610420..1612129	-	1710	WP_000447454.1	DUF927 domain-containing protein	-
IF725_RS08000 (1612143)	1612143..1613012	-	870	WP_001002701.1	primase alpha helix C-terminal domain-containing protein	-
IF725_RS08005 (1613079)	1613079..1613396	-	318	WP_001103933.1	DUF1474 family protein	Toxin
IF725_RS08010 (1613399)	1613399..1613608	-	210	WP_001058488.1	hypothetical protein	Antitoxin
IF725_RS08015 (1613601)	1613601..1613747	-	147	WP_000780975.1	hypothetical protein	-
IF725_RS08020 (1613794)	1613794..1614054	-	261	WP_001147662.1	hypothetical protein	-
IF725_RS08025 (1614056)	1614056..1614253	-	198	WP_000466891.1	helix-turn-helix transcriptional regulator	-
IF725_RS08030 (1614397)	1614397..1615020	+	624	WP_000431539.1	helix-turn-helix transcriptional regulator	-
IF725_RS08035 (1615116)	1615116..1616252	+	1137	WP_000592973.1	site-specific integrase	-
IF725_RS08040 (1616346)	1616346..1617887	-	1542	WP_000424963.1	glutamine-hydrolyzing GMP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sell / sec	1595511..1620684	25173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12511.13 Da
• Isoelectric Point: 4.6364

>T175081 WP_001103933.1 NZ_CP062412:c1613396-1613079 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKEKINDVAIKHGWFVEDKFVKNELETKQEHINFSASYLEHRIQNEHTVELLQVYLKEFGELIQKF
HEIEKASSENFGEESDDAKKLKITE

Nucleotide

• Length: 318 bp

>T175081 NZ_CP081767:322300-322575 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8287.31 Da
• Isoelectric Point: 4.2820

>AT175081 WP_001058488.1 NZ_CP062412:c1613608-1613399 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSIMNNFESYEHTEYVDGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT175081 NZ_CP081767:322572-322931 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: K7ZRX2

Antitoxin

• AlphaFold DB: A0A2X2M557