Detailed information of TA system TA175077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 128979..129755
• Replicon: chromosome
• Accession: NZ_CP062412
• Organism: Staphylococcus aureus strain NAS_AN_181

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF725_RS00815
• Protein ID: WP_000031108.1
• Coordinates: 129603..129755 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: IF725_RS00810
• Protein ID: WP_001251229.1
• Coordinates: 128979..129578 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF725_RS00790 (124896)	124896..126353	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF725_RS00795 (126346)	126346..127068	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF725_RS00800 (127219)	127219..128346	+	1128	WP_029160281.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF725_RS00805 (128351)	128351..128821	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF725_RS00810 (128979)	128979..129578	+	600	WP_001251229.1	glucosamine-6-phosphate isomerase	Antitoxin
IF725_RS00815 (129603)	129603..129755	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF725_RS00820 (130589)	130589..130984	+	396	WP_000901023.1	hypothetical protein	-
IF725_RS00825 (131180)	131180..132565	+	1386	WP_000116231.1	class II fumarate hydratase	-
IF725_RS00830 (133018)	133018..133839	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T175077 WP_000031108.1 NZ_CP062412:129603-129755 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T175077 NZ_CP081764:c44811-44506 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGTTGCTATCGTCTGTTCGTGGATGTACAGAGTGACATCATCGATAC
CCCCGGGCGGCGCATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTTTCGGATAAGGTCTCCCGTGATCTCTATCCGGTGG
TGCACATCGGGGACGACAGCTATCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCTGTCACCGGGGAGGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22329.45 Da
• Isoelectric Point: 5.1445

>AT175077 WP_001251229.1 NZ_CP062412:128979-129578 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEGLGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT175077 NZ_CP081764:c45031-44813 [Escherichia coli]
ATGAAGCAGCGCATCACCGTCACAATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATATCCGGTCT
GGTGAACACGGCCATGCAGAATGAGGCCCGCCGTTTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGAAGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6