Detailed information of TA system TA175072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2691217..2691746
• Replicon: chromosome
• Accession: NZ_CP062411
• Organism: Staphylococcus aureus strain NAS_AN_204

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF728_RS13390
• Protein ID: WP_000621175.1
• Coordinates: 2691384..2691746 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF728_RS13385
• Protein ID: WP_000948331.1
• Coordinates: 2691217..2691387 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF728_RS13355 (2686254)	2686254..2686814	+	561	WP_031894921.1	K(+)-transporting ATPase subunit C	-
IF728_RS13360 (2687022)	2687022..2687501	+	480	WP_001287087.1	hypothetical protein	-
IF728_RS13365 (2687494)	2687494..2689077	+	1584	WP_001294626.1	PH domain-containing protein	-
IF728_RS13370 (2689064)	2689064..2689555	+	492	WP_001205912.1	PH domain-containing protein	-
IF728_RS13375 (2689559)	2689559..2689918	+	360	WP_000581197.1	holo-ACP synthase	-
IF728_RS13380 (2689984)	2689984..2691132	+	1149	WP_001281154.1	alanine racemase	-
IF728_RS13385 (2691217)	2691217..2691387	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF728_RS13390 (2691384)	2691384..2691746	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF728_RS13395 (2692096)	2692096..2693097	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF728_RS13400 (2693216)	2693216..2693542	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF728_RS13405 (2693544)	2693544..2694023	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF728_RS13410 (2693998)	2693998..2694768	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175072 WP_000621175.1 NZ_CP062411:2691384-2691746 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175072 NZ_CP081762:c4328768-4328418 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175072 WP_000948331.1 NZ_CP062411:2691217-2691387 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175072 NZ_CP081762:c4329013-4328762 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1