Detailed information of TA system TA175055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2708474..2709003
• Replicon: chromosome
• Accession: NZ_CP062410
• Organism: Staphylococcus aureus strain NAS_AN_205

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF729_RS13415
• Protein ID: WP_000621175.1
• Coordinates: 2708641..2709003 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF729_RS13410
• Protein ID: WP_000948331.1
• Coordinates: 2708474..2708644 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF729_RS13380 (2703511)	2703511..2704071	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF729_RS13385 (2704279)	2704279..2704758	+	480	WP_001287087.1	hypothetical protein	-
IF729_RS13390 (2704751)	2704751..2706334	+	1584	WP_240053127.1	PH domain-containing protein	-
IF729_RS13395 (2706321)	2706321..2706812	+	492	WP_001205912.1	PH domain-containing protein	-
IF729_RS13400 (2706816)	2706816..2707175	+	360	WP_000581197.1	holo-ACP synthase	-
IF729_RS13405 (2707241)	2707241..2708389	+	1149	WP_001281154.1	alanine racemase	-
IF729_RS13410 (2708474)	2708474..2708644	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF729_RS13415 (2708641)	2708641..2709003	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF729_RS13420 (2709353)	2709353..2710354	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF729_RS13425 (2710473)	2710473..2710799	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF729_RS13430 (2710801)	2710801..2711280	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF729_RS13435 (2711255)	2711255..2712025	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175055 WP_000621175.1 NZ_CP062410:2708641-2709003 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175055 NZ_CP081762:704538-704852 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175055 WP_000948331.1 NZ_CP062410:2708474-2708644 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175055 NZ_CP081762:704849-705265 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCAAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1