Detailed information of TA system TA175024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 121025..121801
• Replicon: chromosome
• Accession: NZ_CP062409
• Organism: Staphylococcus aureus strain NAS_AN_239

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF734_RS00775
• Protein ID: WP_000031108.1
• Coordinates: 121649..121801 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: IF734_RS00770
• Protein ID: WP_001251224.1
• Coordinates: 121025..121624 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF734_RS00750 (116940)	116940..118397	+	1458	WP_000649908.1	ABC transporter permease subunit	-
IF734_RS00755 (118390)	118390..119113	+	724	Protein_119	amino acid ABC transporter ATP-binding protein	-
IF734_RS00760 (119264)	119264..120391	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF734_RS00765 (120396)	120396..120866	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF734_RS00770 (121025)	121025..121624	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
IF734_RS00775 (121649)	121649..121801	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF734_RS00780 (122349)	122349..122744	+	396	WP_000901023.1	hypothetical protein	-
IF734_RS00785 (122940)	122940..124325	+	1386	WP_000116228.1	class II fumarate hydratase	-
IF734_RS00790 (124778)	124778..125599	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T175024 WP_000031108.1 NZ_CP062409:121649-121801 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T175024 NZ_CP081742:320305-320580 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT175024 WP_001251224.1 NZ_CP062409:121025-121624 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT175024 NZ_CP081742:320577-320936 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCACAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4