Detailed information of TA system TA175020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2749550..2750079
• Replicon: chromosome
• Accession: NZ_CP062408
• Organism: Staphylococcus aureus strain NAS_AN_247

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF735_RS13640
• Protein ID: WP_000621175.1
• Coordinates: 2749717..2750079 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF735_RS13635
• Protein ID: WP_000948331.1
• Coordinates: 2749550..2749720 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF735_RS13605 (2744586)	2744586..2745146	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
IF735_RS13610 (2745355)	2745355..2745834	+	480	WP_001287090.1	hypothetical protein	-
IF735_RS13615 (2745827)	2745827..2747410	+	1584	WP_001294626.1	PH domain-containing protein	-
IF735_RS13620 (2747397)	2747397..2747888	+	492	WP_001205910.1	PH domain-containing protein	-
IF735_RS13625 (2747892)	2747892..2748251	+	360	WP_000581197.1	holo-ACP synthase	-
IF735_RS13630 (2748317)	2748317..2749465	+	1149	WP_001281142.1	alanine racemase	-
IF735_RS13635 (2749550)	2749550..2749720	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF735_RS13640 (2749717)	2749717..2750079	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF735_RS13645 (2750428)	2750428..2751429	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF735_RS13650 (2751548)	2751548..2751874	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF735_RS13655 (2751876)	2751876..2752355	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF735_RS13660 (2752330)	2752330..2753100	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175020 WP_000621175.1 NZ_CP062408:2749717-2750079 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175020 NZ_CP081726:c53447-53142 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGTTGCTATCGTCTGTTCGTGGATGTACAGAGTGACATCATCGATAC
CCCCGGGCGGCGCATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTTTCGGATAAGGTCTCCCGTGATCTCTATCCGGTGG
TGCACATCGGGGACGACAGCTATCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCTGTCACCGGGGAGGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175020 WP_000948331.1 NZ_CP062408:2749550-2749720 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175020 NZ_CP081726:c53667-53449 [Escherichia coli]
ATGAAGCAGCGCATCACCGTCACAATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATATCCGGTCT
GGTGAACACGGCCATGCAGAATGAGGCCCGCCGTTTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGAAGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1