Detailed information of TA system TA174996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2280325..2280857
• Replicon: chromosome
• Accession: NZ_CP062407
• Organism: Staphylococcus aureus strain NAS_NP_181

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: Q8VLW9
• Locus tag: IF751_RS10980
• Protein ID: WP_001103944.1
• Coordinates: 2280537..2280857 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: IF751_RS10975
• Protein ID: WP_001058486.1
• Coordinates: 2280325..2280534 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF751_RS10940 (2275571)	2275571..2275855	+	285	WP_000917289.1	co-chaperone GroES	-
IF751_RS10945 (2275931)	2275931..2277547	+	1617	WP_020977428.1	chaperonin GroEL	-
IF751_RS10950 (2277616)	2277616..2278788	-	1173	WP_000179346.1	tyrosine-type recombinase/integrase	-
IF751_RS10955 (2278802)	2278802..2279476	-	675	WP_000620857.1	helix-turn-helix transcriptional regulator	-
IF751_RS10960 (2279649)	2279649..2279867	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
IF751_RS10965 (2279872)	2279872..2280189	+	318	WP_020977427.1	helix-turn-helix domain-containing protein	-
IF751_RS10970 (2280186)	2280186..2280332	+	147	WP_000708434.1	hypothetical protein	-
IF751_RS10975 (2280325)	2280325..2280534	+	210	WP_001058486.1	hypothetical protein	Antitoxin
IF751_RS10980 (2280537)	2280537..2280857	+	321	WP_001103944.1	DUF1474 family protein	Toxin
IF751_RS10985 (2280924)	2280924..2281793	+	870	WP_086114945.1	primase alpha helix C-terminal domain-containing protein	-
IF751_RS10990 (2281807)	2281807..2283507	+	1701	WP_000447470.1	DUF927 domain-containing protein	-
IF751_RS10995 (2283837)	2283837..2284217	+	381	WP_020977426.1	hypothetical protein	-
IF751_RS11000 (2284214)	2284214..2284855	+	642	WP_240053004.1	pathogenicity island protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	2275571..2301579	26008

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12636.31 Da
• Isoelectric Point: 4.8519

>T174996 WP_001103944.1 NZ_CP062407:2280537-2280857 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLQVYLKEFDELIQKF
HEIEKASSDVSLATESDDAKKLKITE

Nucleotide

• Length: 321 bp

>T174996 NZ_CP081724:320302-320577 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8252.31 Da
• Isoelectric Point: 4.3718

>AT174996 WP_001058486.1 NZ_CP062407:2280325-2280534 [Staphylococcus aureus]
MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT174996 NZ_CP081724:320574-320933 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCACAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: Q8VLW9