Detailed information of TA system TA174995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2226511..2227040
• Replicon: chromosome
• Accession: NZ_CP062407
• Organism: Staphylococcus aureus strain NAS_NP_181

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF751_RS10700
• Protein ID: WP_000621175.1
• Coordinates: 2226678..2227040 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF751_RS10695
• Protein ID: WP_000948331.1
• Coordinates: 2226511..2226681 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF751_RS10665 (2221547)	2221547..2222107	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
IF751_RS10670 (2222316)	2222316..2222795	+	480	WP_001287083.1	hypothetical protein	-
IF751_RS10675 (2222788)	2222788..2224371	+	1584	WP_001294642.1	PH domain-containing protein	-
IF751_RS10680 (2224358)	2224358..2224849	+	492	WP_001205916.1	PH domain-containing protein	-
IF751_RS10685 (2224853)	2224853..2225212	+	360	WP_000581197.1	holo-ACP synthase	-
IF751_RS10690 (2225278)	2225278..2226426	+	1149	WP_001281148.1	alanine racemase	-
IF751_RS10695 (2226511)	2226511..2226681	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF751_RS10700 (2226678)	2226678..2227040	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF751_RS10705 (2227390)	2227390..2228391	+	1002	WP_020977445.1	PP2C family protein-serine/threonine phosphatase	-
IF751_RS10710 (2228510)	2228510..2228836	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF751_RS10715 (2228838)	2228838..2229317	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF751_RS10720 (2229292)	2229292..2230062	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174995 WP_000621175.1 NZ_CP062407:2226678-2227040 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174995 NZ_CP081723:c777-511 [Escherichia coli]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174995 WP_000948331.1 NZ_CP062407:2226511-2226681 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174995 NZ_CP081723:c985-761 [Escherichia coli]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1