Detailed information of TA system TA174958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1835416..1835945
• Replicon: chromosome
• Accession: NZ_CP062405
• Organism: Staphylococcus aureus strain NAS_NP_209

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF754_RS09340
• Protein ID: WP_224212111.1
• Coordinates: 1835416..1835778 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF754_RS09345
• Protein ID: WP_000948331.1
• Coordinates: 1835775..1835945 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF754_RS09320 (1832395)	1832395..1833165	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
IF754_RS09325 (1833140)	1833140..1833619	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF754_RS09330 (1833621)	1833621..1833947	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF754_RS09335 (1834066)	1834066..1835067	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF754_RS09340 (1835416)	1835416..1835778	-	363	WP_224212111.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF754_RS09345 (1835775)	1835775..1835945	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF754_RS09350 (1836030)	1836030..1837178	-	1149	WP_001281140.1	alanine racemase	-
IF754_RS09355 (1837244)	1837244..1837603	-	360	WP_000581197.1	holo-ACP synthase	-
IF754_RS09360 (1837607)	1837607..1838098	-	492	WP_001286801.1	PH domain-containing protein	-
IF754_RS09365 (1838091)	1838091..1839668	-	1578	WP_001294646.1	PH domain-containing protein	-
IF754_RS09370 (1839661)	1839661..1840140	-	480	WP_001287082.1	hypothetical protein	-
IF754_RS09375 (1840349)	1840349..1840909	-	561	WP_001092414.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13497.80 Da
• Isoelectric Point: 10.1654

>T174958 WP_224212111.1 NZ_CP062405:c1835778-1835416 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVVAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Nucleotide

• Length: 363 bp

>T174958 NZ_CP081713:c4222771-4222484 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174958 WP_000948331.1 NZ_CP062405:c1835945-1835775 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174958 NZ_CP081713:c4222497-4222195 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1