Detailed information of TA system TA174941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1794497..1795026
• Replicon: chromosome
• Accession: NZ_CP062404
• Organism: Staphylococcus aureus strain NAS_NP_229

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF757_RS09000
• Protein ID: WP_224212111.1
• Coordinates: 1794497..1794859 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF757_RS09005
• Protein ID: WP_000948331.1
• Coordinates: 1794856..1795026 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF757_RS08980 (1791476)	1791476..1792246	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
IF757_RS08985 (1792221)	1792221..1792700	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF757_RS08990 (1792702)	1792702..1793028	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF757_RS08995 (1793147)	1793147..1794148	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF757_RS09000 (1794497)	1794497..1794859	-	363	WP_224212111.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF757_RS09005 (1794856)	1794856..1795026	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF757_RS09010 (1795111)	1795111..1796259	-	1149	WP_001281140.1	alanine racemase	-
IF757_RS09015 (1796325)	1796325..1796684	-	360	WP_000581197.1	holo-ACP synthase	-
IF757_RS09020 (1796688)	1796688..1797179	-	492	WP_001286801.1	PH domain-containing protein	-
IF757_RS09025 (1797172)	1797172..1798749	-	1578	WP_001294646.1	PH domain-containing protein	-
IF757_RS09030 (1798742)	1798742..1799221	-	480	WP_001287082.1	hypothetical protein	-
IF757_RS09035 (1799430)	1799430..1799990	-	561	WP_001092414.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13497.80 Da
• Isoelectric Point: 10.1654

>T174941 WP_224212111.1 NZ_CP062404:c1794859-1794497 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVVAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Nucleotide

• Length: 363 bp

>T174941 NZ_CP081713:c661624-661160 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGATGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174941 WP_000948331.1 NZ_CP062404:c1795026-1794856 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174941 NZ_CP081713:c661959-661624 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1