Detailed information of TA system TA174903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2716590..2717119
• Replicon: chromosome
• Accession: NZ_CP062401
• Organism: Staphylococcus aureus strain NAS_OP_098

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF770_RS13495
• Protein ID: WP_000621175.1
• Coordinates: 2716757..2717119 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF770_RS13490
• Protein ID: WP_000948331.1
• Coordinates: 2716590..2716760 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF770_RS13460 (2711627)	2711627..2712187	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF770_RS13465 (2712395)	2712395..2712874	+	480	WP_001287087.1	hypothetical protein	-
IF770_RS13470 (2712867)	2712867..2714450	+	1584	WP_001294626.1	PH domain-containing protein	-
IF770_RS13475 (2714437)	2714437..2714928	+	492	WP_001205912.1	PH domain-containing protein	-
IF770_RS13480 (2714932)	2714932..2715291	+	360	WP_000581197.1	holo-ACP synthase	-
IF770_RS13485 (2715357)	2715357..2716505	+	1149	WP_001281154.1	alanine racemase	-
IF770_RS13490 (2716590)	2716590..2716760	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF770_RS13495 (2716757)	2716757..2717119	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF770_RS13500 (2717469)	2717469..2718470	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF770_RS13505 (2718589)	2718589..2718915	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF770_RS13510 (2718917)	2718917..2719396	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF770_RS13515 (2719371)	2719371..2720141	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174903 WP_000621175.1 NZ_CP062401:2716757-2717119 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174903 NZ_CP081706:716207-716584 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACTCAGGAGGCTACACCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGGGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174903 WP_000948331.1 NZ_CP062401:2716590-2716760 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174903 NZ_CP081706:715797-716156 [Escherichia coli]
TTGTCGAATGAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTACAGGCGGGCAACCGTCTGCATTATCTCGCTGACCGGGCCAACATCACCGGACAGTTCAGCGACG
CTGATTTACGGTATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAAT
CCCTGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1