Detailed information of TA system TA174882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2650248..2650777
• Replicon: chromosome
• Accession: NZ_CP062400
• Organism: Staphylococcus aureus strain NAS_OP_107

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF772_RS13125
• Protein ID: WP_000621175.1
• Coordinates: 2650415..2650777 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF772_RS13120
• Protein ID: WP_000948331.1
• Coordinates: 2650248..2650418 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF772_RS13090 (2645285)	2645285..2645845	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF772_RS13095 (2646054)	2646054..2646533	+	480	WP_001287079.1	hypothetical protein	-
IF772_RS13100 (2646526)	2646526..2648108	+	1583	Protein_2545	PH domain-containing protein	-
IF772_RS13105 (2648095)	2648095..2648586	+	492	WP_001205912.1	PH domain-containing protein	-
IF772_RS13110 (2648590)	2648590..2648949	+	360	WP_000581197.1	holo-ACP synthase	-
IF772_RS13115 (2649015)	2649015..2650163	+	1149	WP_001281154.1	alanine racemase	-
IF772_RS13120 (2650248)	2650248..2650418	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF772_RS13125 (2650415)	2650415..2650777	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF772_RS13130 (2651127)	2651127..2652128	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF772_RS13135 (2652247)	2652247..2652573	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF772_RS13140 (2652575)	2652575..2653054	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF772_RS13145 (2653029)	2653029..2653799	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174882 WP_000621175.1 NZ_CP062400:2650415-2650777 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174882 NZ_CP081701:836552-836836 [Escherichia coli]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174882 WP_000948331.1 NZ_CP062400:2650248-2650418 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174882 NZ_CP081701:836317-836559 [Escherichia coli]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1