Detailed information of TA system TA174867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2113557..2114086
• Replicon: chromosome
• Accession: NZ_CP062399
• Organism: Staphylococcus aureus strain NAS_OP_143

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF777_RS10455
• Protein ID: WP_000621175.1
• Coordinates: 2113557..2113919 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF777_RS10460
• Protein ID: WP_000948331.1
• Coordinates: 2113916..2114086 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF777_RS10435 (IF777_10395)	2110535..2111305	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
IF777_RS10440 (IF777_10400)	2111280..2111759	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF777_RS10445 (IF777_10405)	2111761..2112087	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF777_RS10450 (IF777_10410)	2112206..2113207	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF777_RS10455 (IF777_10415)	2113557..2113919	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF777_RS10460 (IF777_10420)	2113916..2114086	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF777_RS10465 (IF777_10425)	2114171..2115319	-	1149	WP_001281145.1	alanine racemase	-
IF777_RS10470 (IF777_10430)	2115385..2115744	-	360	WP_000581200.1	holo-ACP synthase	-
IF777_RS10475 (IF777_10435)	2115748..2116239	-	492	WP_078103494.1	PH domain-containing protein	-
IF777_RS10480 (IF777_10440)	2116226..2117809	-	1584	WP_045173493.1	PH domain-containing protein	-
IF777_RS10485 (IF777_10445)	2117802..2118281	-	480	WP_001287078.1	hypothetical protein	-
IF777_RS10490 (IF777_10450)	2118490..2119050	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174867 WP_000621175.1 NZ_CP062399:c2113919-2113557 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174867 NZ_CP081698:3502121-3502339 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174867 WP_000948331.1 NZ_CP062399:c2114086-2113916 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174867 NZ_CP081698:3501721-3502095 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1