Detailed information of TA system TA174858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2030966..2031495
• Replicon: chromosome
• Accession: NZ_CP062398
• Organism: Staphylococcus aureus strain NAS_OP_154

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF781_RS10000
• Protein ID: WP_000621175.1
• Coordinates: 2030966..2031328 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF781_RS10005
• Protein ID: WP_000948331.1
• Coordinates: 2031325..2031495 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF781_RS09980 (IF781_09935)	2027944..2028714	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
IF781_RS09985 (IF781_09940)	2028689..2029168	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF781_RS09990 (IF781_09945)	2029170..2029496	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF781_RS09995 (IF781_09950)	2029615..2030616	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF781_RS10000 (IF781_09955)	2030966..2031328	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF781_RS10005 (IF781_09960)	2031325..2031495	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF781_RS10010 (IF781_09965)	2031580..2032728	-	1149	WP_001281154.1	alanine racemase	-
IF781_RS10015 (IF781_09970)	2032794..2033153	-	360	WP_000581197.1	holo-ACP synthase	-
IF781_RS10020 (IF781_09975)	2033157..2033648	-	492	WP_001205912.1	PH domain-containing protein	-
IF781_RS10025 (IF781_09980)	2033635..2035218	-	1584	WP_001294626.1	PH domain-containing protein	-
IF781_RS10030 (IF781_09985)	2035211..2035690	-	480	WP_001287087.1	hypothetical protein	-
IF781_RS10035 (IF781_09990)	2035898..2036458	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174858 WP_000621175.1 NZ_CP062398:c2031328-2030966 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174858 NZ_CP081698:875588-875995 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCTTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174858 WP_000948331.1 NZ_CP062398:c2031495-2031325 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174858 NZ_CP081698:875341-875607 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1