Detailed information of TA system TA174850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2722528..2723066
• Replicon: chromosome
• Accession: NZ_CP062395
• Organism: Staphylococcus aureus strain NAS_AN_005

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: -
• Locus tag: IF694_RS13325
• Protein ID: WP_042907701.1
• Coordinates: 2722740..2723066 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: C5QLS5
• Locus tag: IF694_RS13320
• Protein ID: WP_001058489.1
• Coordinates: 2722528..2722737 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF694_RS13285 (IF694_13140)	2717774..2718058	+	285	WP_031918283.1	co-chaperone GroES	-
IF694_RS13290 (IF694_13145)	2718134..2719750	+	1617	WP_047213652.1	chaperonin GroEL	-
IF694_RS13295 (IF694_13150)	2719819..2720991	-	1173	WP_000179346.1	tyrosine-type recombinase/integrase	-
IF694_RS13300 (IF694_13155)	2721005..2721679	-	675	WP_000620857.1	helix-turn-helix transcriptional regulator	-
IF694_RS13305 (IF694_13160)	2721852..2722070	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
IF694_RS13310 (IF694_13165)	2722075..2722392	+	318	WP_000481967.1	helix-turn-helix domain-containing protein	-
IF694_RS13315 (IF694_13170)	2722389..2722535	+	147	WP_000784885.1	hypothetical protein	-
IF694_RS13320 (IF694_13175)	2722528..2722737	+	210	WP_001058489.1	hypothetical protein	Antitoxin
IF694_RS13325 (IF694_13180)	2722740..2723066	+	327	WP_042907701.1	DUF1474 family protein	Toxin
IF694_RS13330 (IF694_13185)	2723132..2724001	+	870	WP_064138582.1	primase alpha helix C-terminal domain-containing protein	-
IF694_RS13335 (IF694_13190)	2724015..2725715	+	1701	WP_000447470.1	DUF927 domain-containing protein	-
IF694_RS13340 (IF694_13195)	2726045..2726425	+	381	WP_000356942.1	hypothetical protein	-
IF694_RS13345 (IF694_13200)	2726422..2727063	+	642	WP_001047698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12969.58 Da
• Isoelectric Point: 4.8297

>T174850 WP_042907701.1 NZ_CP062395:2722740-2723066 [Staphylococcus aureus]
MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYIHEPNHTLNKVDVIKEGLSYHERRIHNNQMFDLFHLYIEQFDNIIE
KFYEIEKASSDVSLATESDDAQKLKITE

Nucleotide

• Length: 327 bp

>T174850 NZ_CP081691:c31429-31145 [Escherichia coli]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8225.24 Da
• Isoelectric Point: 4.2820

>AT174850 WP_001058489.1 NZ_CP062395:2722528-2722737 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVDGIEVVSEVSREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT174850 NZ_CP081691:c31664-31422 [Escherichia coli]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Structures

Antitoxin

• AlphaFold DB: A0A853V601