Detailed information of TA system TA174849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2670649..2671178
• Replicon: chromosome
• Accession: NZ_CP062395
• Organism: Staphylococcus aureus strain NAS_AN_005

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF694_RS13055
• Protein ID: WP_000621175.1
• Coordinates: 2670816..2671178 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF694_RS13050
• Protein ID: WP_000948331.1
• Coordinates: 2670649..2670819 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF694_RS13020 (IF694_12875)	2665685..2666245	+	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-
IF694_RS13025 (IF694_12880)	2666454..2666933	+	480	WP_001287075.1	hypothetical protein	-
IF694_RS13030 (IF694_12885)	2666926..2668509	+	1584	WP_240022138.1	PH domain-containing protein	-
IF694_RS13035 (IF694_12890)	2668496..2668987	+	492	WP_001205919.1	PH domain-containing protein	-
IF694_RS13040 (IF694_12895)	2668991..2669350	+	360	WP_000581198.1	holo-ACP synthase	-
IF694_RS13045 (IF694_12900)	2669416..2670564	+	1149	WP_001281143.1	alanine racemase	-
IF694_RS13050 (IF694_12905)	2670649..2670819	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF694_RS13055 (IF694_12910)	2670816..2671178	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF694_RS13060 (IF694_12915)	2671528..2672529	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF694_RS13065 (IF694_12920)	2672648..2672974	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF694_RS13070 (IF694_12925)	2672976..2673455	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF694_RS13075 (IF694_12930)	2673430..2674200	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174849 WP_000621175.1 NZ_CP062395:2670816-2671178 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174849 NZ_CP081691:28036-28452 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174849 WP_000948331.1 NZ_CP062395:2670649-2670819 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174849 NZ_CP081691:27809-28039 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1