Detailed information of TA system TA174837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2714655..2715184
• Replicon: chromosome
• Accession: NZ_CP062393
• Organism: Staphylococcus aureus strain NAS_AN_009

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF695_RS13490
• Protein ID: WP_000621175.1
• Coordinates: 2714822..2715184 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF695_RS13485
• Protein ID: WP_000948331.1
• Coordinates: 2714655..2714825 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF695_RS13455 (2709692)	2709692..2710252	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF695_RS13460 (2710460)	2710460..2710939	+	480	WP_049308993.1	membrane protein	-
IF695_RS13465 (2710932)	2710932..2712515	+	1584	WP_001294626.1	PH domain-containing protein	-
IF695_RS13470 (2712502)	2712502..2712993	+	492	WP_001205910.1	PH domain-containing protein	-
IF695_RS13475 (2712997)	2712997..2713356	+	360	WP_000581200.1	holo-ACP synthase	-
IF695_RS13480 (2713422)	2713422..2714570	+	1149	WP_001281145.1	alanine racemase	-
IF695_RS13485 (2714655)	2714655..2714825	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF695_RS13490 (2714822)	2714822..2715184	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF695_RS13495 (2715534)	2715534..2716535	+	1002	WP_240025740.1	PP2C family protein-serine/threonine phosphatase	-
IF695_RS13500 (2716654)	2716654..2716980	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF695_RS13505 (2716982)	2716982..2717461	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF695_RS13510 (2717436)	2717436..2718206	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174837 WP_000621175.1 NZ_CP062393:2714822-2715184 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174837 NZ_CP081690:2241995-2242098 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174837 WP_000948331.1 NZ_CP062393:2714655-2714825 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174837 NZ_CP081690:c2242136-2241865 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1