Detailed information of TA system TA174824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 121955..122731
• Replicon: chromosome
• Accession: NZ_CP062393
• Organism: Staphylococcus aureus strain NAS_AN_009

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF695_RS00775
• Protein ID: WP_000031108.1
• Coordinates: 122579..122731 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: IF695_RS00770
• Protein ID: WP_049309001.1
• Coordinates: 121955..122554 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF695_RS00750 (117871)	117871..119328	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF695_RS00755 (119321)	119321..120043	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF695_RS00760 (120194)	120194..121321	+	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF695_RS00765 (121326)	121326..121796	+	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF695_RS00770 (121955)	121955..122554	+	600	WP_049309001.1	glucosamine-6-phosphate isomerase	Antitoxin
IF695_RS00775 (122579)	122579..122731	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF695_RS00780 (123508)	123508..123903	+	396	WP_000901021.1	hypothetical protein	-
IF695_RS00785 (124099)	124099..125484	+	1386	WP_000116224.1	class II fumarate hydratase	-
IF695_RS00790 (125947)	125947..126768	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174824 WP_000031108.1 NZ_CP062393:122579-122731 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174824 NZ_CP081685:c221699-221283 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22371.53 Da
• Isoelectric Point: 5.1445

>AT174824 WP_049309001.1 NZ_CP062393:121955-122554 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQVPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174824 NZ_CP081685:c221926-221696 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6