Detailed information of TA system TA174819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2699749..2700278
• Replicon: chromosome
• Accession: NZ_CP062390
• Organism: Staphylococcus aureus strain NAS_AN_036

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF699_RS13385
• Protein ID: WP_000621175.1
• Coordinates: 2699916..2700278 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF699_RS13380
• Protein ID: WP_000948331.1
• Coordinates: 2699749..2699919 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF699_RS13350 (2694785)	2694785..2695345	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF699_RS13355 (2695554)	2695554..2696033	+	480	WP_001287079.1	hypothetical protein	-
IF699_RS13360 (2696026)	2696026..2697609	+	1584	WP_001294620.1	PH domain-containing protein	-
IF699_RS13365 (2697596)	2697596..2698087	+	492	WP_240024558.1	PH domain-containing protein	-
IF699_RS13370 (2698091)	2698091..2698450	+	360	WP_000581197.1	holo-ACP synthase	-
IF699_RS13375 (2698516)	2698516..2699664	+	1149	WP_001281154.1	alanine racemase	-
IF699_RS13380 (2699749)	2699749..2699919	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF699_RS13385 (2699916)	2699916..2700278	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF699_RS13390 (2700628)	2700628..2701629	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF699_RS13395 (2701748)	2701748..2702074	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF699_RS13400 (2702076)	2702076..2702555	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF699_RS13405 (2702530)	2702530..2703300	+	771	WP_240024559.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174819 WP_000621175.1 NZ_CP062390:2699916-2700278 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174819 NZ_CP081684:c4256186-4255899 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174819 WP_000948331.1 NZ_CP062390:2699749-2699919 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174819 NZ_CP081684:c4255912-4255610 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1