Detailed information of TA system TA174801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2664219..2664748
• Replicon: chromosome
• Accession: NZ_CP062388
• Organism: Staphylococcus aureus strain NAS_AN_044

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF700_RS13135
• Protein ID: WP_000621175.1
• Coordinates: 2664386..2664748 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF700_RS13130
• Protein ID: WP_000948331.1
• Coordinates: 2664219..2664389 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF700_RS13100 (2659256)	2659256..2659816	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF700_RS13105 (2660024)	2660024..2660503	+	480	WP_001287087.1	hypothetical protein	-
IF700_RS13110 (2660496)	2660496..2662079	+	1584	WP_001294626.1	PH domain-containing protein	-
IF700_RS13115 (2662066)	2662066..2662557	+	492	WP_001205912.1	PH domain-containing protein	-
IF700_RS13120 (2662561)	2662561..2662920	+	360	WP_000581197.1	holo-ACP synthase	-
IF700_RS13125 (2662986)	2662986..2664134	+	1149	WP_001281154.1	alanine racemase	-
IF700_RS13130 (2664219)	2664219..2664389	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF700_RS13135 (2664386)	2664386..2664748	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF700_RS13140 (2665097)	2665097..2666098	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF700_RS13145 (2666217)	2666217..2666543	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF700_RS13150 (2666545)	2666545..2667024	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF700_RS13155 (2666999)	2666999..2667769	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174801 WP_000621175.1 NZ_CP062388:2664386-2664748 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174801 NZ_CP081682:1505-2542 [Escherichia coli]
ATGGCACAGAACCCATTTAAAGCACTAAATATTAATACAGACAAGATTGAAGCTGCCTTGACGCAGAATGGCGTCACAAA
CTTTTCTTCTATCGTGAGAAACGAAAGAGAAACACATTTATCAGGGACATATAAAGAAATAGATTTTTTAATAAAGCTAA
TGCCATCAGGTGGAAACACCACTATAGGAAGGGCATCAGGACAAAACAACACTTACTTTGATGAGATCGCTGGCATTATA
AAAGAAAACTGTTTGTATTCAGATAAAAAAACGTTTGAATACACAATCCCAAGATTCAGCGATGAAAATAAGGAAATGTT
GTTTGCATTTCTTACTGAAGAAAACGTAAAAATAGAAGAAGATAAAAACAACGACCCCAACTGTAAACATCAATACATCA
TGACCACTGGCAATGGTGACAGAGTCAGAGCAAAAATTTACAACCGTGGCTCAATTCAATTCCAAGGTAAATATCTTCAA
ATCGCAAGTTTGATTAATGATTTCATGTGCACCATACTGGACATGAAAGAGATTATAGAACAGAAGAATCAAGAATTTAA
TGTTGACATAAAAAAAGAAACAATTGAGTCAGAATTACATAGTAAACTACCAAAAAGCATCGACCTAATCCATGACGACA
TCAAAAAACAGCTATCCTGCTCGCTCATAATGAAACAAATAGATGTCGAAATGGAAGATTACTCAACATATTGTTTCTCA
GCCTTAAGATCCATAGAAGGCTTTATATATCAACTACTTGGAAGCGTATGCAACCCATCATCATCAAGGAATCTTGGCGA
GTACTTCACTGAGAATAACCCGAAATACATAATAAGAGATATACACCAAGAAACTATTAATGGTGAAATAGCAGAAGTTT
TGTGTGAGTGCTACACTTACTGGCATGAAAACAGACATGGCTTGTTCCATATGAAACCAGGTGTGGCTGACACTAAGACA
ATTGACAAAGCAGAATCTATTGAAATTATCGATACTGTATGTCAGTTGATAGATAAAGGCATGGCTAGGTTGAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174801 WP_000948331.1 NZ_CP062388:2664219-2664389 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174801 NZ_CP081682:2542-2922 [Escherichia coli]
ATGAAAATAAACAAAAAATATCAAATAGAGGTGATAAAGGATAAAGATAAAACTTTGTTCATTGTCTACGCTACAGATAT
TTATAGCCCGAGCGAATTTTTCTCTAAAATCGAGTCTGATTTAAAGAAAAAGAAAGTTGGTGGCGATGTTTTTTTTGATT
TAATAATTCCTAATGGTGGAAAAAAAGACCGTTACGTCCATACATCATTCAACGGCGAGCGATTGACAAGCTACACATTA
AACAAAGTTACGGAAACAGATAAGTATAAATCCTTATCGGAACGCTCAGCTTCGTTCTTTAAGAATAACTTTAATAATAT
TAATGCAAACCTTCTATCTAAAGCCACATCATTTGCTTTAAAACAAGGTATTCCAATATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1