Detailed information of TA system TA174783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2670644..2671173
• Replicon: chromosome
• Accession: NZ_CP062386
• Organism: Staphylococcus aureus strain NAS_AN_047

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF701_RS13120
• Protein ID: WP_000621175.1
• Coordinates: 2670811..2671173 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF701_RS13115
• Protein ID: WP_000948331.1
• Coordinates: 2670644..2670814 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF701_RS13085 (2665680)	2665680..2666240	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
IF701_RS13090 (2666449)	2666449..2666928	+	480	WP_001287090.1	hypothetical protein	-
IF701_RS13095 (2666921)	2666921..2668504	+	1584	WP_001294626.1	PH domain-containing protein	-
IF701_RS13100 (2668491)	2668491..2668982	+	492	WP_001205910.1	PH domain-containing protein	-
IF701_RS13105 (2668986)	2668986..2669345	+	360	WP_000581197.1	holo-ACP synthase	-
IF701_RS13110 (2669411)	2669411..2670559	+	1149	WP_001281142.1	alanine racemase	-
IF701_RS13115 (2670644)	2670644..2670814	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF701_RS13120 (2670811)	2670811..2671173	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF701_RS13125 (2671522)	2671522..2672523	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF701_RS13130 (2672642)	2672642..2672968	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF701_RS13135 (2672970)	2672970..2673449	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF701_RS13140 (2673424)	2673424..2674194	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174783 WP_000621175.1 NZ_CP062386:2670811-2671173 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174783 NZ_CP081677:1968764-1969018 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174783 WP_000948331.1 NZ_CP062386:2670644-2670814 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174783 NZ_CP081677:1968516-1968767 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1