Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2650918..2651432 Replicon chromosome
Accession NZ_CP062386
Organism Staphylococcus aureus strain NAS_AN_047

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag IF701_RS13030 Protein ID WP_000581792.1
Coordinates 2651223..2651432 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag IF701_RS13025 Protein ID WP_000138932.1
Coordinates 2650918..2651211 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF701_RS13005 (2646522) 2646522..2647163 + 642 WP_000483153.1 thiamine phosphate synthase -
IF701_RS13010 (2647249) 2647249..2648121 + 873 WP_000725802.1 membrane protein insertase YidC -
IF701_RS13015 (2648593) 2648593..2649240 - 648 WP_001187630.1 HD domain-containing protein -
IF701_RS13020 (2649268) 2649268..2650752 - 1485 WP_000571549.1 cardiolipin synthase -
IF701_RS13025 (2650918) 2650918..2651211 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
IF701_RS13030 (2651223) 2651223..2651432 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
IF701_RS13035 (2651560) 2651560..2651697 + 138 WP_000828354.1 Lmo0850 family protein -
IF701_RS13040 (2651961) 2651961..2653163 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
IF701_RS13045 (2653481) 2653481..2654551 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
IF701_RS13050 (2654566) 2654566..2655924 + 1359 WP_000611470.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T174782 WP_000581792.1 NZ_CP062386:2651223-2651432 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T174782 NZ_CP081677:1968516-1968767 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT174782 WP_000138932.1 NZ_CP062386:2650918-2651211 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT174782 NZ_CP081677:1968764-1969018 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References