Detailed information of TA system TA174770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 128920..129696
• Replicon: chromosome
• Accession: NZ_CP062386
• Organism: Staphylococcus aureus strain NAS_AN_047

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF701_RS00810
• Protein ID: WP_000031108.1
• Coordinates: 129544..129696 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: IF701_RS00805
• Protein ID: WP_001251229.1
• Coordinates: 128920..129519 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF701_RS00785 (124837)	124837..126294	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF701_RS00790 (126287)	126287..127009	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF701_RS00795 (127160)	127160..128287	+	1128	WP_240022486.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF701_RS00800 (128292)	128292..128762	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF701_RS00805 (128920)	128920..129519	+	600	WP_001251229.1	glucosamine-6-phosphate isomerase	Antitoxin
IF701_RS00810 (129544)	129544..129696	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF701_RS00815 (130530)	130530..130925	+	396	WP_000901023.1	hypothetical protein	-
IF701_RS00820 (131121)	131121..132506	+	1386	WP_000116231.1	class II fumarate hydratase	-
IF701_RS00825 (132959)	132959..133780	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174770 WP_000031108.1 NZ_CP062386:129544-129696 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174770 NZ_CP081506:62990-63331 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22329.45 Da
• Isoelectric Point: 5.1445

>AT174770 WP_001251229.1 NZ_CP062386:128920-129519 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEGLGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174770 NZ_CP081506:62760-62990 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6