Detailed information of TA system TA174755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2788781..2789310
• Replicon: chromosome
• Accession: NZ_CP062382
• Organism: Staphylococcus aureus strain NAS_AN_068

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF703_RS13940
• Protein ID: WP_000621175.1
• Coordinates: 2788948..2789310 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF703_RS13935
• Protein ID: WP_000948331.1
• Coordinates: 2788781..2788951 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF703_RS13905 (2783817)	2783817..2784377	+	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-
IF703_RS13910 (2784586)	2784586..2785065	+	480	WP_001287075.1	hypothetical protein	-
IF703_RS13915 (2785058)	2785058..2786641	+	1584	WP_001294640.1	PH domain-containing protein	-
IF703_RS13920 (2786628)	2786628..2787119	+	492	WP_001205919.1	PH domain-containing protein	-
IF703_RS13925 (2787123)	2787123..2787482	+	360	WP_000581198.1	holo-ACP synthase	-
IF703_RS13930 (2787548)	2787548..2788696	+	1149	WP_001281143.1	alanine racemase	-
IF703_RS13935 (2788781)	2788781..2788951	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF703_RS13940 (2788948)	2788948..2789310	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF703_RS13945 (2789660)	2789660..2790661	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF703_RS13950 (2790780)	2790780..2791106	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF703_RS13955 (2791108)	2791108..2791587	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF703_RS13960 (2791562)	2791562..2792332	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174755 WP_000621175.1 NZ_CP062382:2788948-2789310 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174755 NZ_CP081490:6137459-6137749 [Pseudomonas protegens]
ATGCACACCCCAGTGACCCGCTTCGACGCGGCAGAGTACCTCAAGACCCCGGAAGACATGGTGGCCTACCTCGACGCCTG
CTTCGAGGAAGATGCCGGCGACGGCAAGCTGATCCGCGCCGCCCTCAACGACATCGCCCGCGCCCGGGGCATGACCCAGG
TGGCCCGCGACACCGGCCTGGGTCGAGAGAGTCTGTACAAGGCCCTGGGCAGCCAGGGCAATCCCGAGTTCGCGACTATC
ATCAAGGTCATGAAGGCACTGGGACTCAAGCTCCACGTCAGCCCGGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174755 WP_000948331.1 NZ_CP062382:2788781-2788951 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174755 NZ_CP081490:6137153-6137455 [Pseudomonas protegens]
ATGTACGTCTTCGAGCAGACCCTGGAGTTCAGCCGCTGGCTCCTGCACCTGAAGGATCCGCAAGGCAAAACCCGTGTACT
GGCCAGGATCCGCGCCGCCCAACTGGGCCGATTCGGCGACTGCGAACCGGTAGGCGAAGGTGTCTATGAAATGCGCATCC
ATCGCGGCCCCGGCTATCGGGTGTACTTCTCGCGCCGGAACACCGTGATCTACCTGTTGTTGATCGGCGGTGACAAGGGC
ACCCAGAGACGCGATATCCAACGCGCCAGGCAACTGGCCCACGACCTCAAGAGCGAGGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1