Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 131260..132036 Replicon chromosome
Accession NZ_CP062382
Organism Staphylococcus aureus strain NAS_AN_068

Toxin (Protein)


Gene name tsbT Uniprot ID A0A380DXA2
Locus tag IF703_RS00820 Protein ID WP_000031110.1
Coordinates 131884..132036 (+) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID A0A0D1IYN8
Locus tag IF703_RS00815 Protein ID WP_001251219.1
Coordinates 131260..131859 (+) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF703_RS00795 (127177) 127177..128634 + 1458 WP_031927464.1 ABC transporter permease subunit -
IF703_RS00800 (128627) 128627..129348 + 722 Protein_128 amino acid ABC transporter ATP-binding protein -
IF703_RS00805 (129499) 129499..130626 + 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
IF703_RS00810 (130631) 130631..131101 + 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
IF703_RS00815 (131260) 131260..131859 + 600 WP_001251219.1 glucosamine-6-phosphate isomerase Antitoxin
IF703_RS00820 (131884) 131884..132036 + 153 WP_000031110.1 SAS053 family protein Toxin
IF703_RS00825 (132696) 132696..133091 + 396 WP_000901023.1 hypothetical protein -
IF703_RS00830 (133287) 133287..134672 + 1386 WP_000116225.1 class II fumarate hydratase -
IF703_RS00835 (135123) 135123..135944 - 822 WP_000669377.1 RluA family pseudouridine synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5964.34 Da        Isoelectric Point: 3.9953

>T174741 WP_000031110.1 NZ_CP062382:131884-132036 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMERISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T174741 NZ_CP081489:c3524996-3524661 [Escherichia coli BL21(DE3)]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22357.50 Da        Isoelectric Point: 5.1445

>AT174741 WP_001251219.1 NZ_CP062382:131260-131859 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNIEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT174741 NZ_CP081489:c3525244-3524996 [Escherichia coli BL21(DE3)]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A380DXA2

Antitoxin

Source ID Structure
AlphaFold DB A0A0D1IYN8

References