Detailed information of TA system TA174711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1763681..1764210
• Replicon: chromosome
• Accession: NZ_CP062378
• Organism: Staphylococcus aureus strain NAS_AN_079

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF705_RS08895
• Protein ID: WP_000621175.1
• Coordinates: 1763681..1764043 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF705_RS08900
• Protein ID: WP_000948331.1
• Coordinates: 1764040..1764210 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF705_RS08875 (1760659)	1760659..1761429	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
IF705_RS08880 (1761404)	1761404..1761883	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF705_RS08885 (1761885)	1761885..1762211	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF705_RS08890 (1762330)	1762330..1763331	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF705_RS08895 (1763681)	1763681..1764043	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF705_RS08900 (1764040)	1764040..1764210	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF705_RS08905 (1764295)	1764295..1765443	-	1149	WP_001281154.1	alanine racemase	-
IF705_RS08910 (1765509)	1765509..1765868	-	360	WP_000581197.1	holo-ACP synthase	-
IF705_RS08915 (1765872)	1765872..1766363	-	492	WP_001205912.1	PH domain-containing protein	-
IF705_RS08920 (1766350)	1766350..1767933	-	1584	WP_001294626.1	PH domain-containing protein	-
IF705_RS08925 (1767926)	1767926..1768405	-	480	WP_001287087.1	hypothetical protein	-
IF705_RS08930 (1768613)	1768613..1769173	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174711 WP_000621175.1 NZ_CP062378:c1764043-1763681 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174711 NZ_CP081457:4669040-4669348 [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
ATGAACACACTCATCAGAACCCACGAATTCGACGCATGGCTGAAAGGCTTGAGGGACGTAACAGCGCGCCTGAGAATCCT
GAACCGTATCATGGCGGCACAGGAAGGCAACTTTGGTGACTGTGAGCCAGTCGGGCACGGCATCAGTGAAATGCGCATCT
TCGTAGGACCTGGCTACCGTGTGTACTACACCAGGCGCGCAGAAGTGGTCTATCTGCTACTGTGTGGCGGCAACAAGTCC
TCACAGAAGCGCGACATAAAGCTGGCGAAATCCATCCTCGATGAGATCGAGAAAGGAGACGTAGAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174711 WP_000948331.1 NZ_CP062378:c1764210-1764040 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174711 NZ_CP081457:4669345-4669626 [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
ATGAGAGAGTTGAAGGTTTTCGAAGCTTCTGACTACCTGAACAACGACGAGGAAATTGCCGAGTTTCTGAGCATTTCCCT
GGAAGATCCAAACCCTGACGTTTTCCTCAGGGCTTTGCAGGAAGTCGCGAAGGCGCGTGGAATGAGCCAGCTTGCCCGTG
ACGCTGGCATCAATCGCGAATCGCTCTACAAAGCTTTGACACCAGGTGCAAAGCCTCGGTATGACACGGTTTTGAAGGTG
ATGAATGCGTTAGGCATGAAGCTGACCGCTCAGCACGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1