Detailed information of TA system TA174689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 120912..121688
• Replicon: chromosome
• Accession: NZ_CP062376
• Organism: Staphylococcus aureus strain NAS_AN_099

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF707_RS00780
• Protein ID: WP_000031108.1
• Coordinates: 121536..121688 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: IF707_RS00775
• Protein ID: WP_001251224.1
• Coordinates: 120912..121511 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF707_RS00755 (116828)	116828..118285	+	1458	WP_117184657.1	ABC transporter permease subunit	-
IF707_RS00760 (118278)	118278..119000	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF707_RS00765 (119151)	119151..120278	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF707_RS00770 (120283)	120283..120753	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF707_RS00775 (120912)	120912..121511	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
IF707_RS00780 (121536)	121536..121688	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF707_RS00785 (122291)	122291..122686	+	396	WP_000901023.1	hypothetical protein	-
IF707_RS00790 (122882)	122882..124267	+	1386	WP_000116229.1	class II fumarate hydratase	-
IF707_RS00795 (124730)	124730..125551	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174689 WP_000031108.1 NZ_CP062376:121536-121688 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174689 NZ_CP081351:c1172204-1171965 [Klebsiella michiganensis]
ATGGTCGGTTTAATATTTTATATGTATTATTATGATACTGGTAAACACAATTCGCCTCATGTTCATGTTAAATACAGCAG
TCATGAACTTATTGTTGATATAAATACAGGAGTTGAAATAGTAGGATACTTACCAAATAAACAAAGAAAAATGGCATTGT
TACATATAAAAGTGAATAAGAGTAAGTTACTGAAAATGTGGAAGTTAGCAGTTAATGGTAATAACCCAGGACATTTGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT174689 WP_001251224.1 NZ_CP062376:120912-121511 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174689 NZ_CP081351:c1171953-1171450 [Klebsiella michiganensis]
ATGAACAAAATAATAGATGTGGATGTTATAGGTAAATTTGAGTTGAAATTAACCTATGAAAACGGGACGGTTTGTGAGGT
TGACCTTTACAAATATAACGATCAGCCTCCTTTCACTGATCCAAAAAAATTCGTCAAATTTGGATTACTTCCTAATGGTA
ATTTAGAATGGATTCCGAATGTTACAATATCCTCTGAGGCACTGCTGCAACGAGGAAGTAACATCACTCATGACGAATCT
ATTAAGAAAACAAATTTTGCTAATATAATTTCTATGGCTCTTTATGATGCAATTATAGAAAACAGACCAGAAATTATCCA
AGCTGCACTTAGGACTGCCGTTGACAAATATGGCACAACGAAAATAGCTAAAAATTCCGGCACAAAAAGTAGAACAAGTA
TTTATAAATCTCTCGATGAGAATACATATGTTAGCATGTCTACCGTTGTAAATTTAGCTCATGCCGTTTTGGATTTAGAA
GAAAAAGAAAGCATTCTTCGTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4