Detailed information of TA system TA174674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2639661..2640175
• Replicon: chromosome
• Accession: NZ_CP062370
• Organism: Staphylococcus aureus strain NAS_AN_111

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: IF711_RS13015
• Protein ID: WP_000581792.1
• Coordinates: 2639966..2640175 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: IF711_RS13010
• Protein ID: WP_000138932.1
• Coordinates: 2639661..2639954 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF711_RS12990 (2634824)	2634824..2635465	+	642	WP_000483153.1	thiamine phosphate synthase	-
IF711_RS12995 (2635551)	2635551..2636423	+	873	WP_000725802.1	membrane protein insertase YidC	-
IF711_RS13000 (2637336)	2637336..2637983	-	648	WP_001187630.1	HD domain-containing protein	-
IF711_RS13005 (2638011)	2638011..2639495	-	1485	WP_000571549.1	cardiolipin synthase	-
IF711_RS13010 (2639661)	2639661..2639954	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
IF711_RS13015 (2639966)	2639966..2640175	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
IF711_RS13020 (2640303)	2640303..2640440	+	138	WP_000828354.1	Lmo0850 family protein	-
IF711_RS13025 (2640704)	2640704..2641906	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
IF711_RS13030 (2642224)	2642224..2643294	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
IF711_RS13035 (2643309)	2643309..2644667	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T174674 WP_000581792.1 NZ_CP062370:2639966-2640175 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T174674 NZ_CP081345:c4214146-4213571 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT174674 WP_000138932.1 NZ_CP062370:2639661-2639954 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT174674 NZ_CP081345:c4214613-4214143 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13