Detailed information of TA system TA174665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1153231..1153708
• Replicon: chromosome
• Accession: NZ_CP062370
• Organism: Staphylococcus aureus strain NAS_AN_111

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF711_RS05750
• Protein ID: WP_000052076.1
• Coordinates: 1153520..1153708 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF711_RS05745
• Protein ID: WP_001059690.1
• Coordinates: 1153231..1153491 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF711_RS05710 (1148624)	1148624..1149340	-	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-
IF711_RS05715 (1149488)	1149488..1149910	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
IF711_RS05720 (1150309)	1150309..1150803	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF711_RS05725 (1150958)	1150958..1151575	+	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
IF711_RS05730 (1151648)	1151648..1152229	-	582	WP_001140693.1	histidine phosphatase family protein	-
IF711_RS05735 (1152454)	1152454..1152690	+	237	WP_000349581.1	hypothetical protein	-
IF711_RS05740 (1152687)	1152687..1152890	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF711_RS05745 (1153231)	1153231..1153491	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF711_RS05750 (1153520)	1153520..1153708	+	189	WP_000052076.1	hypothetical protein	Toxin
IF711_RS05755 (1153896)	1153896..1154465	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
IF711_RS05760 (1154553)	1154553..1154837	+	285	WP_001004399.1	hypothetical protein	-
IF711_RS05765 (1154899)	1154899..1155117	+	219	WP_000005886.1	hypothetical protein	-
IF711_RS05770 (1155609)	1155609..1155809	-	201	WP_001059082.1	cold-shock protein	-
IF711_RS05775 (1156166)	1156166..1156852	-	687	WP_000141557.1	thermonuclease family protein	-
IF711_RS05780 (1157193)	1157193..1157714	-	522	WP_000824482.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T174665 WP_000052076.1 NZ_CP062370:1153520-1153708 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174665 NZ_CP081315:c108659-108351 [Citrobacter farmeri]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174665 WP_001059690.1 NZ_CP062370:1153231-1153491 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174665 NZ_CP081315:c108901-108662 [Citrobacter farmeri]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56