Detailed information of TA system TA174658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2660714..2661243
• Replicon: chromosome
• Accession: NZ_CP062368
• Organism: Staphylococcus aureus strain NAS_AN_115

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF712_RS13090
• Protein ID: WP_000621175.1
• Coordinates: 2660881..2661243 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF712_RS13085
• Protein ID: WP_000948331.1
• Coordinates: 2660714..2660884 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF712_RS13055 (2655751)	2655751..2656311	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF712_RS13060 (2656519)	2656519..2656998	+	480	WP_001287087.1	hypothetical protein	-
IF712_RS13065 (2656991)	2656991..2658574	+	1584	WP_001294626.1	PH domain-containing protein	-
IF712_RS13070 (2658561)	2658561..2659052	+	492	WP_001205912.1	PH domain-containing protein	-
IF712_RS13075 (2659056)	2659056..2659415	+	360	WP_000581197.1	holo-ACP synthase	-
IF712_RS13080 (2659481)	2659481..2660629	+	1149	WP_001281154.1	alanine racemase	-
IF712_RS13085 (2660714)	2660714..2660884	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF712_RS13090 (2660881)	2660881..2661243	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF712_RS13095 (2661593)	2661593..2662594	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF712_RS13100 (2662713)	2662713..2663039	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF712_RS13105 (2663041)	2663041..2663520	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF712_RS13110 (2663495)	2663495..2664265	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174658 WP_000621175.1 NZ_CP062368:2660881-2661243 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174658 NZ_CP081314:c4132050-4131643 [Citrobacter farmeri]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCGCACAGTGGCTGTCGCTACTGCTGCATGGGCTGGTGGCTGC
GGCTATTCTATTAATGCCATGGCCGCTGAGTTACACGCCGCTGTGGTTGATACTGCTTTCGCTGGTGGTGTTTGACTGTG
TTCGCAGCCAGCGGCGAATTAACGCCCGTCAGGGAGAGGTAAAACTTCTGATGGATGGACGGCTGCGCTGGCAGGGGCAG
GACTGGCATATCGTCAGTGCCCCGTGGATGATCAAAACTGGCATGATGCTCCGTTTGCGTTCAGAAACCGGTAAACGCCA
GCATTTGTGGCTCGCGGCAGACAGTATGGATGAGGCCGAATGGCGGGATTTACGCCGCCTGATGATGCAAAAGTCGACGC
TGGGATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174658 WP_000948331.1 NZ_CP062368:2660714-2660884 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174658 NZ_CP081314:c4132297-4132031 [Citrobacter farmeri]
ATGGATATTAACAATAAAGCACGTATTCACTGGGCATGCCGACGCGGTATGCGTGAACTCGATATTTCCATCATGCCGTT
CTTCGAACATGAATACGACACGTTGAGCGATGATGAAAAACGTATTTTCATTCGTCTTCTGGAGTGCGATGACCCGGATT
TATTCAACTGGCTGATGAATCACGGTAAACCGGCAGATGCTGAACTGGAGCAGATGGTACGACTCATCCAGACACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1