Detailed information of TA system TA174648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1153138..1153615
• Replicon: chromosome
• Accession: NZ_CP062368
• Organism: Staphylococcus aureus strain NAS_AN_115

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF712_RS05735
• Protein ID: WP_000052076.1
• Coordinates: 1153427..1153615 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF712_RS05730
• Protein ID: WP_001059690.1
• Coordinates: 1153138..1153398 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF712_RS05695 (1148531)	1148531..1149247	-	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-
IF712_RS05700 (1149395)	1149395..1149817	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
IF712_RS05705 (1150216)	1150216..1150710	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF712_RS05710 (1150865)	1150865..1151482	+	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
IF712_RS05715 (1151555)	1151555..1152136	-	582	WP_001140693.1	histidine phosphatase family protein	-
IF712_RS05720 (1152361)	1152361..1152597	+	237	WP_000349581.1	hypothetical protein	-
IF712_RS05725 (1152594)	1152594..1152797	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF712_RS05730 (1153138)	1153138..1153398	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF712_RS05735 (1153427)	1153427..1153615	+	189	WP_000052076.1	hypothetical protein	Toxin
IF712_RS05740 (1153803)	1153803..1154372	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
IF712_RS05745 (1154460)	1154460..1154744	+	285	WP_001004399.1	hypothetical protein	-
IF712_RS05750 (1154806)	1154806..1155024	+	219	WP_000005886.1	hypothetical protein	-
IF712_RS05755 (1155516)	1155516..1155716	-	201	WP_001059082.1	cold-shock protein	-
IF712_RS05760 (1156073)	1156073..1156759	-	687	WP_000141557.1	thermonuclease family protein	-
IF712_RS05765 (1157100)	1157100..1157621	-	522	WP_000824482.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T174648 WP_000052076.1 NZ_CP062368:1153427-1153615 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174648 NZ_CP081307:83752-84168 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174648 WP_001059690.1 NZ_CP062368:1153138-1153398 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174648 NZ_CP081307:83525-83755 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56