Detailed information of TA system TA174616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2658695..2659224
• Replicon: chromosome
• Accession: NZ_CP062362
• Organism: Staphylococcus aureus strain NAS_AN_152

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF721_RS13090
• Protein ID: WP_000621175.1
• Coordinates: 2658862..2659224 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF721_RS13085
• Protein ID: WP_000948331.1
• Coordinates: 2658695..2658865 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF721_RS13055 (2653731)	2653731..2654291	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
IF721_RS13060 (2654500)	2654500..2654979	+	480	WP_001287090.1	hypothetical protein	-
IF721_RS13065 (2654972)	2654972..2656555	+	1584	WP_001294626.1	PH domain-containing protein	-
IF721_RS13070 (2656542)	2656542..2657033	+	492	WP_001205910.1	PH domain-containing protein	-
IF721_RS13075 (2657037)	2657037..2657396	+	360	WP_000581197.1	holo-ACP synthase	-
IF721_RS13080 (2657462)	2657462..2658610	+	1149	WP_001281142.1	alanine racemase	-
IF721_RS13085 (2658695)	2658695..2658865	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF721_RS13090 (2658862)	2658862..2659224	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF721_RS13095 (2659574)	2659574..2660575	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF721_RS13100 (2660694)	2660694..2661020	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF721_RS13105 (2661022)	2661022..2661501	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF721_RS13110 (2661476)	2661476..2662246	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174616 WP_000621175.1 NZ_CP062362:2658862-2659224 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174616 NZ_CP081257:3472549-3472830 [Phaeobacter inhibens]
ATGATCCGAAGCACCAAGGGCAAACTCGCTGCCAATGCCGTGAAAGGCAAGTATGGCAAAGGCTTCCCAGGTGACTTGGT
GAAGCGCACGCGGGCTATGCTTTCGGCGCTGGATGCTGCGGTTGTTGTCGAAGATTTGCGGTTCCCGCCCGGCAACCACC
TAGAAGAGCTGAAAGGGGATCGGGCCGGGCAACACTCCGTGCGGATCAACGGGCAATGGCGCATCTGCTTTGTCTGGACG
CCGAACGGCCCTGCTGACGTCGAGATCGTCGATTACCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174616 WP_000948331.1 NZ_CP062362:2658695-2658865 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174616 NZ_CP081257:3472842-3473132 [Phaeobacter inhibens]
ATGAGCCTGCTTGAAGAACCTATGCACCCCGGTGAAGTCCTGAAAGAGCTTTACCTTGATCCTCTGGACATGGGGGCAAT
CGCGTTTGCCCGGCGTCTGGACGTGCCTCGGACGCGGATCGAGCGGTTGATCAAGGGCACGACTGGGATCACGCCTGATA
CTGCGCTGCGCCTCGCTCGTGTTTTCAACACTATTCCGGCTTATTGGGTGAACTTACAGACGAATTTCGACATGGCAGTT
GCGGCCAAAGAAATCGACGTTTCTAACATTGAACCACTCATTGCCGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1