Detailed information of TA system TA174605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1161322..1161799
• Replicon: chromosome
• Accession: NZ_CP062362
• Organism: Staphylococcus aureus strain NAS_AN_152

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF721_RS05775
• Protein ID: WP_000052076.1
• Coordinates: 1161611..1161799 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF721_RS05770
• Protein ID: WP_001059690.1
• Coordinates: 1161322..1161582 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF721_RS05735 (1156715)	1156715..1157431	-	717	WP_000150012.1	type I 3-dehydroquinate dehydratase	-
IF721_RS05740 (1157579)	1157579..1158001	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
IF721_RS05745 (1158400)	1158400..1158894	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF721_RS05750 (1159049)	1159049..1159666	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
IF721_RS05755 (1159739)	1159739..1160320	-	582	WP_001140693.1	histidine phosphatase family protein	-
IF721_RS05760 (1160545)	1160545..1160781	+	237	WP_000368687.1	hypothetical protein	-
IF721_RS05765 (1160778)	1160778..1160981	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF721_RS05770 (1161322)	1161322..1161582	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF721_RS05775 (1161611)	1161611..1161799	+	189	WP_000052076.1	hypothetical protein	Toxin
IF721_RS05780 (1161987)	1161987..1162556	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
IF721_RS05785 (1162644)	1162644..1162928	+	285	WP_001004395.1	hypothetical protein	-
IF721_RS05790 (1162990)	1162990..1163208	+	219	WP_000005886.1	hypothetical protein	-
IF721_RS05795 (1163704)	1163704..1163904	-	201	WP_001059082.1	cold-shock protein	-
IF721_RS05800 (1164261)	1164261..1164947	-	687	WP_001792695.1	thermonuclease family protein	-
IF721_RS05805 (1165288)	1165288..1165809	-	522	WP_000824484.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T174605 WP_000052076.1 NZ_CP062362:1161611-1161799 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174605 NZ_CP081202:6700031-6700309 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGTTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174605 WP_001059690.1 NZ_CP062362:1161322-1161582 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174605 NZ_CP081202:6700321-6700626 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56