Detailed information of TA system TA174593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2077871..2078400
• Replicon: chromosome
• Accession: NZ_CP062360
• Organism: Staphylococcus aureus strain NAS_AN_157

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF722_RS10300
• Protein ID: WP_000621175.1
• Coordinates: 2077871..2078233 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF722_RS10305
• Protein ID: WP_000948331.1
• Coordinates: 2078230..2078400 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF722_RS10280 (2074850)	2074850..2075620	-	771	WP_072491781.1	RNA polymerase sigma factor SigB	-
IF722_RS10285 (2075595)	2075595..2076074	-	480	WP_098818138.1	anti-sigma B factor RsbW	-
IF722_RS10290 (2076076)	2076076..2076402	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF722_RS10295 (2076521)	2076521..2077522	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF722_RS10300 (2077871)	2077871..2078233	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF722_RS10305 (2078230)	2078230..2078400	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF722_RS10310 (2078485)	2078485..2079633	-	1149	WP_072491783.1	alanine racemase	-
IF722_RS10315 (2079699)	2079699..2080058	-	360	WP_000581198.1	holo-ACP synthase	-
IF722_RS10320 (2080062)	2080062..2080553	-	492	WP_001205919.1	PH domain-containing protein	-
IF722_RS10325 (2080540)	2080540..2082123	-	1584	WP_072491784.1	PH domain-containing protein	-
IF722_RS10330 (2082116)	2082116..2082595	-	480	WP_072491785.1	hypothetical protein	-
IF722_RS10335 (2082804)	2082804..2083364	-	561	WP_063652345.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174593 WP_000621175.1 NZ_CP062360:c2078233-2077871 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174593 NZ_CP081201:c1488208-1487957 [Pseudomonas phytophila]
ATGTCCAAGCAAGAAAAGCTGCTCGCCAAGCTACTGAACAGGCAATCGGGATTTAGCTGGCCAGAACTGGTCGCGCTGTT
GAAAGGTTTCGGCTACAAGCAAATCGAGGGTGATGGCAGCCGCGTCAAATTTGATAACGGCAATGCTCAAGCGATGATAA
ACCTGCATCGCCCCCATCCAGGCAATGAGATAAAGGCATACGTTAAACGACAGGTTATCGAGCATCTGAAAGCGGGAGGT
CTGATTTCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174593 WP_000948331.1 NZ_CP062360:c2078400-2078230 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174593 NZ_CP081201:c1487960-1487619 [Pseudomonas phytophila]
ATGAGTACGCTGTTACAGCACCGGGGCTATTACGGTTCAATTGAAGCCAGCCCTGAAGATAACTGCCTGTTCGGAAAACT
TCAGTTCATACGCGCACTGGTGAACTATGAAGGCGAAACCGTGGCGGAGCTATCTCAATCCTTTCGTAACGCGGTAGATG
ACTATCTGGATACTTGCTCAACTCTCGGGCAAGACCCGGAATTGCCCTGCAAAGGCTCGTTCAATGTCAGGGTCGGGCAT
GACCTGCATTTGGCAGCCAGCGTTGCCGCGACCCGCGATAAAATTTCACTTAATGACCTGACACGTAAAGCGCTTCGAGA
ATATCTGAGTCACCATGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1