Detailed information of TA system TA174584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 401081..401598
• Replicon: chromosome
• Accession: NZ_CP062360
• Organism: Staphylococcus aureus strain NAS_AN_157

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: -
• Locus tag: IF722_RS01850
• Protein ID: WP_240025275.1
• Coordinates: 401293..401598 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: A0A2B8BQK3
• Locus tag: IF722_RS01845
• Protein ID: WP_001058490.1
• Coordinates: 401081..401290 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF722_RS01805 (396560)	396560..396802	+	243	WP_000897044.1	30S ribosomal protein S18	-
IF722_RS01810 (397041)	397041..397940	-	900	WP_001797289.1	Abi family protein	-
IF722_RS01815 (397942)	397942..399151	-	1210	Protein_358	tyrosine-type recombinase/integrase	-
IF722_RS01820 (399177)	399177..400100	-	924	WP_050436923.1	helix-turn-helix transcriptional regulator	-
IF722_RS01825 (400207)	400207..400407	+	201	WP_001033058.1	helix-turn-helix domain-containing protein	-
IF722_RS01830 (400446)	400446..400658	+	213	WP_033863184.1	helix-turn-helix transcriptional regulator	-
IF722_RS01835 (400659)	400659..400930	+	272	Protein_362	helix-turn-helix domain-containing protein	-
IF722_RS01840 (400942)	400942..401088	+	147	WP_000784873.1	hypothetical protein	-
IF722_RS01845 (401081)	401081..401290	+	210	WP_001058490.1	hypothetical protein	Antitoxin
IF722_RS01850 (401293)	401293..401598	+	306	WP_240025275.1	DUF1474 family protein	Toxin
IF722_RS01855 (401673)	401673..403286	+	1614	WP_106875302.1	phage resistance protein	-
IF722_RS01860 (403457)	403457..404260	+	804	WP_033863177.1	bifunctional DNA primase/polymerase	-
IF722_RS01865 (404247)	404247..404519	+	273	WP_001149387.1	hypothetical protein	-
IF722_RS01870 (404521)	404521..405162	+	642	WP_072490956.1	pathogenicity island protein	-
IF722_RS01875 (405611)	405611..405952	+	342	WP_001190614.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	396005..416670	20665

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12172.46 Da
• Isoelectric Point: 4.4054

>T174584 WP_240025275.1 NZ_CP062360:401293-401598 [Staphylococcus aureus]
MNLEIKDLFSDLKLLKDRFEDLKDNHGWHFEELYPHEPNHVLNKDELIGEGFSYHERRIHNNQMFDLFHIYIEQFDNIIE
KFYEIEKASSDVSLATESDDA

Nucleotide

• Length: 306 bp

>T174584 NZ_CP081200:182701-182982 [Pseudomonas sp. 3-2]
ATGACCTATAAGCTCGAATTTCTGCCTTCCGCTCACAAAGAGTGGAACAAACTGGGTCATACGTTGCGTGATCAATTTAA
AAAGAAGCTGGCTGAGCGTCTTGAGCGTCCCCGTATTCCGGCTGACTCGCTCCACGGCATGCCTGATTGCTACAAGATCA
AACTCAAAGCGTCCGGTTATCGACTGGTTTACCAAGTGATCGAAGAGCGGGTGGTTGTCTCGGTAGTCGCCGTGGGTAAG
CGCGAGCGAAGCGACGTCTACGAAAACGCGAAAAGACGCTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8153.17 Da
• Isoelectric Point: 4.3591

>AT174584 WP_001058490.1 NZ_CP062360:401081-401290 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVDGIEVVSEVSREKHLELIMKWCAQELKNNFQLGKGE

Nucleotide

• Length: 210 bp

>AT174584 NZ_CP081200:182466-182711 [Pseudomonas sp. 3-2]
ATGCAAAGTGTTCTGGCCGATATGGCTGTCAGTGTTTCTGAATTGAAGAAAAACCCCTCCGCAGTGCTAAACGGCGCGCA
CGGAGGAGCAGTTGCTGTCCTGAATCACAACCGGGTCATGGGCTACATGGTGCCGGCTGACGTTTACGAAGCCATGATGG
AGCGGCTCGATGATCTCGAGTTGGCAGAAATCGTTCGTTCTCGCAGTCACGAGACTCCCATACCGGTAAGCCTGGATGAC
CTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2B8BQK3