Detailed information of TA system TA174565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2647541..2648070
• Replicon: chromosome
• Accession: NZ_CP062356
• Organism: Staphylococcus aureus strain NAS_AN_203

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF727_RS13020
• Protein ID: WP_000621175.1
• Coordinates: 2647708..2648070 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF727_RS13015
• Protein ID: WP_000948331.1
• Coordinates: 2647541..2647711 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF727_RS12985 (2642578)	2642578..2643138	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF727_RS12990 (2643346)	2643346..2643825	+	480	WP_001287087.1	hypothetical protein	-
IF727_RS12995 (2643818)	2643818..2645401	+	1584	WP_001294626.1	PH domain-containing protein	-
IF727_RS13000 (2645388)	2645388..2645879	+	492	WP_001205912.1	PH domain-containing protein	-
IF727_RS13005 (2645883)	2645883..2646242	+	360	WP_000581197.1	holo-ACP synthase	-
IF727_RS13010 (2646308)	2646308..2647456	+	1149	WP_001281154.1	alanine racemase	-
IF727_RS13015 (2647541)	2647541..2647711	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF727_RS13020 (2647708)	2647708..2648070	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF727_RS13025 (2648420)	2648420..2649421	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF727_RS13030 (2649540)	2649540..2649866	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF727_RS13035 (2649868)	2649868..2650347	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF727_RS13040 (2650322)	2650322..2651092	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174565 WP_000621175.1 NZ_CP062356:2647708-2648070 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174565 NZ_CP081192:1888717-1888971 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174565 WP_000948331.1 NZ_CP062356:2647541-2647711 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174565 NZ_CP081192:1888469-1888720 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1