Detailed information of TA system TA174548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2659308..2659837
• Replicon: chromosome
• Accession: NZ_CP062354
• Organism: Staphylococcus aureus strain NAS_AN_206

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF730_RS13115
• Protein ID: WP_000621175.1
• Coordinates: 2659475..2659837 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF730_RS13110
• Protein ID: WP_000948331.1
• Coordinates: 2659308..2659478 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF730_RS13080 (2654344)	2654344..2654904	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF730_RS13085 (2655113)	2655113..2655592	+	480	WP_001287079.1	hypothetical protein	-
IF730_RS13090 (2655585)	2655585..2657168	+	1584	WP_001294620.1	PH domain-containing protein	-
IF730_RS13095 (2657155)	2657155..2657646	+	492	WP_001205912.1	PH domain-containing protein	-
IF730_RS13100 (2657650)	2657650..2658009	+	360	WP_000581197.1	holo-ACP synthase	-
IF730_RS13105 (2658075)	2658075..2659223	+	1149	WP_001281154.1	alanine racemase	-
IF730_RS13110 (2659308)	2659308..2659478	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF730_RS13115 (2659475)	2659475..2659837	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF730_RS13120 (2660187)	2660187..2661188	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF730_RS13125 (2661307)	2661307..2661633	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF730_RS13130 (2661635)	2661635..2662114	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF730_RS13135 (2662089)	2662089..2662859	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174548 WP_000621175.1 NZ_CP062354:2659475-2659837 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174548 NZ_CP081189:3453252-3453470 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174548 WP_000948331.1 NZ_CP062354:2659308-2659478 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174548 NZ_CP081189:3452850-3453224 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1