Detailed information of TA system TA174512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2654300..2654829
• Replicon: chromosome
• Accession: NZ_CP062350
• Organism: Staphylococcus aureus strain NAS_AN_250

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF736_RS13195
• Protein ID: WP_000621175.1
• Coordinates: 2654467..2654829 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF736_RS13190
• Protein ID: WP_000948331.1
• Coordinates: 2654300..2654470 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF736_RS13160 (2649336)	2649336..2649896	+	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-
IF736_RS13165 (2650105)	2650105..2650584	+	480	WP_031590204.1	hypothetical protein	-
IF736_RS13170 (2650577)	2650577..2652160	+	1584	WP_001294626.1	PH domain-containing protein	-
IF736_RS13175 (2652147)	2652147..2652638	+	492	WP_072353916.1	PH domain-containing protein	-
IF736_RS13180 (2652642)	2652642..2653001	+	360	WP_000581197.1	holo-ACP synthase	-
IF736_RS13185 (2653067)	2653067..2654215	+	1149	WP_001281154.1	alanine racemase	-
IF736_RS13190 (2654300)	2654300..2654470	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF736_RS13195 (2654467)	2654467..2654829	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF736_RS13200 (2655178)	2655178..2656179	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF736_RS13205 (2656298)	2656298..2656624	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF736_RS13210 (2656626)	2656626..2657105	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF736_RS13215 (2657080)	2657080..2657850	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174512 WP_000621175.1 NZ_CP062350:2654467-2654829 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174512 NZ_CP081110:18463-18765 [Sulfitobacter mediterraneus]
ATGAAGCCTGTGCATCTGTCACCCGCCGCTCAGGCAGATGTCGAGGACATTTGGGACTACACAGAAACGACCTGGGGCTC
TGCTCAAGCCGAAAAGTATATCGGAGACATCCAGCAAACCTGCCACGGTGTTGCGACAGGCAGAGCAATTGTTCGATCTG
CGGAGAGCATTCGAGCAGGCTATCAACGGTCAAATGTTGGTTCCCATGTGCTTTACTTCAAAGAGCTTGAAGACCGGTTC
GTGATCGTTCGGATCTTGCACCAGAAAATGGATGTTGGCCGATACATTGCAGAGCGGAGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174512 WP_000948331.1 NZ_CP062350:2654300-2654470 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174512 NZ_CP081110:18221-18466 [Sulfitobacter mediterraneus]
ATGGGTCGAAACACGTCGGTCTCCCTTGGGGACCATTTCACGAACTTTATAGATGAGCGCTTGGATACCGGGCGATACAA
CTCGGCCAGTGATGTCGTGCGGGCTGGTTTGCGTCTTCTTGAAGAACAGGAAACCCAGCTGGAGGCTCTCCGCGCTGCTC
TGATTGAAGGCGAGCTTTCTGGCGAACCAGCCCCATTTGATTTTAATGGACTTGTTGCCCGTAAGAAGTCAGCCAACCCC
AAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1