Detailed information of TA system TA174499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 128923..129699
• Replicon: chromosome
• Accession: NZ_CP062350
• Organism: Staphylococcus aureus strain NAS_AN_250

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF736_RS00815
• Protein ID: WP_000031108.1
• Coordinates: 129547..129699 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: IF736_RS00810
• Protein ID: WP_001251230.1
• Coordinates: 128923..129522 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF736_RS00790 (124839)	124839..126296	+	1458	WP_000649908.1	ABC transporter permease subunit	-
IF736_RS00795 (126289)	126289..127011	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF736_RS00800 (127162)	127162..128289	+	1128	WP_000379988.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF736_RS00805 (128294)	128294..128764	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF736_RS00810 (128923)	128923..129522	+	600	WP_001251230.1	hypothetical protein	Antitoxin
IF736_RS00815 (129547)	129547..129699	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF736_RS00820 (130301)	130301..130696	+	396	WP_000901023.1	hypothetical protein	-
IF736_RS00825 (130892)	130892..132277	+	1386	WP_000116229.1	class II fumarate hydratase	-
IF736_RS00830 (132729)	132729..133550	-	822	WP_240018954.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174499 WP_000031108.1 NZ_CP062350:129547-129699 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174499 NZ_CP081016:c1046057-1045689 [Pseudomonas sp. 2hn]
ATGGAATCGGACAGGCAACCCAAGCCGCTTTACTGGGTCGCAAGCAGCAAAAAGGATCTGCTGTGCCTCCCCGAGGACGT
CCAGCAAACCGTCGGCTTTGCTCTGCACCTGGCTCAGACAGGAGGCAGGCACCTACAGGCTAAAGTCCTAAGAGGATTTT
CAGGCGCTGGCGTGCTCGAAGTCGTGGAAGACCATGACGGCAACGCCTATCGCGCGGTCTATACCGTACGCCTTGGCACT
GCGGTGTATGTCTTGCATTGCTTCCAGAAGAAGTCCAAGGCGGGCATCAGGACGCCCGAAACAGACATCGCCCTGGTGCG
CAAGCGATTGAAGCTTGCACAGGCCCACTCAGAAGGAATCCAGGCATGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22369.51 Da
• Isoelectric Point: 4.9728

>AT174499 WP_001251230.1 NZ_CP062350:128923-129522 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKYAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174499 NZ_CP081016:c1045692-1045372 [Pseudomonas sp. 2hn]
ATGAGCGAAATCGAACAGGGCAGCGGCAATGTCTACGCAGACCTCAACTACCCGGATGCAGACGAGATGCTGGTCAAGGC
ACGCCTGGCGAGCAAGATCTGCAGCATCATCAAGGCCCGCCATCTGACCCAGATGCAAGCCGCGCAAATCCTTGGCCTGC
CTCAGCCAAAAGTATCGGAAATGATTCGCGGAAAATTCCGTGGTATCAGCGAAAGCAAGATGATCGACTGCCTGGCACGC
CTTGGCCGCGACGTGCAGATCGTGGTCAAGGCCGCACCGCGTTCACGTCCCCAAGGGCGGGTGGAGGTCATCTTCGCCTG
A

Structures

Toxin

• AlphaFold DB: X5E2E6