Detailed information of TA system TA174486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1852932..1853461
• Replicon: chromosome
• Accession: NZ_CP062348
• Organism: Staphylococcus aureus strain NAS_AN_260

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF738_RS09430
• Protein ID: WP_000621175.1
• Coordinates: 1852932..1853294 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF738_RS09435
• Protein ID: WP_000948331.1
• Coordinates: 1853291..1853461 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF738_RS09410 (1849910)	1849910..1850680	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
IF738_RS09415 (1850655)	1850655..1851134	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
IF738_RS09420 (1851136)	1851136..1851462	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF738_RS09425 (1851581)	1851581..1852582	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF738_RS09430 (1852932)	1852932..1853294	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF738_RS09435 (1853291)	1853291..1853461	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF738_RS09440 (1853546)	1853546..1854694	-	1149	WP_001281154.1	alanine racemase	-
IF738_RS09445 (1854760)	1854760..1855119	-	360	WP_000581197.1	holo-ACP synthase	-
IF738_RS09450 (1855123)	1855123..1855614	-	492	WP_001205912.1	PH domain-containing protein	-
IF738_RS09455 (1855601)	1855601..1857184	-	1584	WP_001294626.1	PH domain-containing protein	-
IF738_RS09460 (1857177)	1857177..1857656	-	480	WP_240024482.1	hypothetical protein	-
IF738_RS09465 (1857865)	1857865..1858425	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174486 WP_000621175.1 NZ_CP062348:c1853294-1852932 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174486 NZ_CP081010:c4419383-4419006 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174486 WP_000948331.1 NZ_CP062348:c1853461-1853291 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174486 NZ_CP081010:c4419841-4419473 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1