Detailed information of TA system TA174477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2729166..2729695
• Replicon: chromosome
• Accession: NZ_CP062346
• Organism: Staphylococcus aureus strain NAS_AN_261

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF739_RS13485
• Protein ID: WP_000621175.1
• Coordinates: 2729333..2729695 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF739_RS13480
• Protein ID: WP_000948331.1
• Coordinates: 2729166..2729336 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF739_RS13450 (2724202)	2724202..2724762	+	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-
IF739_RS13455 (2724971)	2724971..2725450	+	480	WP_001287075.1	hypothetical protein	-
IF739_RS13460 (2725443)	2725443..2727026	+	1584	WP_001294640.1	PH domain-containing protein	-
IF739_RS13465 (2727013)	2727013..2727504	+	492	WP_001205919.1	PH domain-containing protein	-
IF739_RS13470 (2727508)	2727508..2727867	+	360	WP_000581198.1	holo-ACP synthase	-
IF739_RS13475 (2727933)	2727933..2729081	+	1149	WP_001281143.1	alanine racemase	-
IF739_RS13480 (2729166)	2729166..2729336	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF739_RS13485 (2729333)	2729333..2729695	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF739_RS13490 (2730045)	2730045..2731046	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF739_RS13495 (2731165)	2731165..2731491	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF739_RS13500 (2731493)	2731493..2731972	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF739_RS13505 (2731947)	2731947..2732717	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174477 WP_000621175.1 NZ_CP062346:2729333-2729695 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174477 NZ_CP081010:2594546-2594824 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174477 WP_000948331.1 NZ_CP062346:2729166-2729336 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174477 NZ_CP081010:2594824-2595108 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1