Detailed information of TA system TA174461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2716152..2716681
• Replicon: chromosome
• Accession: NZ_CP062344
• Organism: Staphylococcus aureus strain NAS_AN_265

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF740_RS13490
• Protein ID: WP_000621175.1
• Coordinates: 2716319..2716681 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF740_RS13485
• Protein ID: WP_000948331.1
• Coordinates: 2716152..2716322 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF740_RS13455 (2711188)	2711188..2711748	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF740_RS13460 (2711957)	2711957..2712436	+	480	WP_001287088.1	hypothetical protein	-
IF740_RS13465 (2712429)	2712429..2714012	+	1584	WP_001294626.1	PH domain-containing protein	-
IF740_RS13470 (2713999)	2713999..2714490	+	492	WP_001205910.1	PH domain-containing protein	-
IF740_RS13475 (2714494)	2714494..2714853	+	360	WP_000581200.1	holo-ACP synthase	-
IF740_RS13480 (2714919)	2714919..2716067	+	1149	WP_001281145.1	alanine racemase	-
IF740_RS13485 (2716152)	2716152..2716322	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF740_RS13490 (2716319)	2716319..2716681	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF740_RS13495 (2717031)	2717031..2718032	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF740_RS13500 (2718151)	2718151..2718477	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF740_RS13505 (2718479)	2718479..2718958	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
IF740_RS13510 (2718933)	2718933..2719703	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174461 WP_000621175.1 NZ_CP062344:2716319-2716681 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174461 NZ_CP081009:c4190435-4190094 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174461 WP_000948331.1 NZ_CP062344:2716152-2716322 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174461 NZ_CP081009:c4190773-4190456 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1