Detailed information of TA system TA174450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1202776..1203253
• Replicon: chromosome
• Accession: NZ_CP062344
• Organism: Staphylococcus aureus strain NAS_AN_265

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF740_RS06070
• Protein ID: WP_000052076.1
• Coordinates: 1203065..1203253 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF740_RS06065
• Protein ID: WP_001059690.1
• Coordinates: 1202776..1203036 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF740_RS06030 (1198169)	1198169..1198885	-	717	WP_000150018.1	type I 3-dehydroquinate dehydratase	-
IF740_RS06035 (1199033)	1199033..1199455	+	423	WP_000974455.1	organic hydroperoxide resistance protein	-
IF740_RS06040 (1199854)	1199854..1200348	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF740_RS06045 (1200503)	1200503..1201120	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
IF740_RS06050 (1201193)	1201193..1201774	-	582	WP_239794378.1	histidine phosphatase family protein	-
IF740_RS06055 (1201999)	1201999..1202235	+	237	WP_000368687.1	hypothetical protein	-
IF740_RS06060 (1202232)	1202232..1202435	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF740_RS06065 (1202776)	1202776..1203036	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF740_RS06070 (1203065)	1203065..1203253	+	189	WP_000052076.1	hypothetical protein	Toxin
IF740_RS06075 (1203441)	1203441..1204010	+	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
IF740_RS06080 (1204098)	1204098..1204382	+	285	WP_001004399.1	hypothetical protein	-
IF740_RS06085 (1204444)	1204444..1204662	+	219	WP_000005884.1	hypothetical protein	-
IF740_RS06090 (1205157)	1205157..1205357	-	201	WP_001059082.1	cold-shock protein	-
IF740_RS06095 (1205714)	1205714..1206400	-	687	WP_000141557.1	thermonuclease family protein	-
IF740_RS06100 (1206741)	1206741..1207262	-	522	WP_000824484.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T174450 WP_000052076.1 NZ_CP062344:1203065-1203253 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174450 NZ_CP081009:3026224-3026400 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174450 WP_001059690.1 NZ_CP062344:1202776-1203036 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174450 NZ_CP081009:3026446-3026862 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56