Detailed information of TA system TA174440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2704409..2704938
• Replicon: chromosome
• Accession: NZ_CP062342
• Organism: Staphylococcus aureus strain NAS_AN_270

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF741_RS13410
• Protein ID: WP_000621175.1
• Coordinates: 2704576..2704938 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF741_RS13405
• Protein ID: WP_000948331.1
• Coordinates: 2704409..2704579 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF741_RS13375 (2699445)	2699445..2700005	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF741_RS13380 (2700214)	2700214..2700693	+	480	WP_001287079.1	hypothetical protein	-
IF741_RS13385 (2700686)	2700686..2702269	+	1584	WP_001294620.1	PH domain-containing protein	-
IF741_RS13390 (2702256)	2702256..2702747	+	492	WP_001205912.1	PH domain-containing protein	-
IF741_RS13395 (2702751)	2702751..2703110	+	360	WP_000581197.1	holo-ACP synthase	-
IF741_RS13400 (2703176)	2703176..2704324	+	1149	WP_001281154.1	alanine racemase	-
IF741_RS13405 (2704409)	2704409..2704579	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF741_RS13410 (2704576)	2704576..2704938	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF741_RS13415 (2705288)	2705288..2706289	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF741_RS13420 (2706408)	2706408..2706734	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF741_RS13425 (2706736)	2706736..2707215	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF741_RS13430 (2707190)	2707190..2707960	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174440 WP_000621175.1 NZ_CP062342:2704576-2704938 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174440 NZ_CP081009:68114-68217 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174440 WP_000948331.1 NZ_CP062342:2704409-2704579 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174440 NZ_CP081009:c68255-67984 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1