Detailed information of TA system TA174430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1198479..1198956
• Replicon: chromosome
• Accession: NZ_CP062342
• Organism: Staphylococcus aureus strain NAS_AN_270

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF741_RS06070
• Protein ID: WP_000052076.1
• Coordinates: 1198768..1198956 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF741_RS06065
• Protein ID: WP_001059690.1
• Coordinates: 1198479..1198739 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF741_RS06030 (1193893)	1193893..1194609	-	717	WP_000150019.1	type I 3-dehydroquinate dehydratase	-
IF741_RS06035 (1194757)	1194757..1195179	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
IF741_RS06040 (1195557)	1195557..1196051	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF741_RS06045 (1196206)	1196206..1196823	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
IF741_RS06050 (1196896)	1196896..1197477	-	582	WP_001140697.1	histidine phosphatase family protein	-
IF741_RS06055 (1197702)	1197702..1197938	+	237	WP_000368687.1	hypothetical protein	-
IF741_RS06060 (1197935)	1197935..1198138	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF741_RS06065 (1198479)	1198479..1198739	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF741_RS06070 (1198768)	1198768..1198956	+	189	WP_000052076.1	hypothetical protein	Toxin
IF741_RS06075 (1199144)	1199144..1199713	+	570	WP_001106652.1	lipoprotein N-acylation protein LnsA	-
IF741_RS06080 (1199801)	1199801..1200085	+	285	WP_001004394.1	hypothetical protein	-
IF741_RS06085 (1200147)	1200147..1200372	+	226	Protein_1176	hypothetical protein	-
IF741_RS06090 (1200867)	1200867..1201067	-	201	WP_001059082.1	cold-shock protein	-
IF741_RS06095 (1201424)	1201424..1202110	-	687	WP_001566557.1	thermonuclease family protein	-
IF741_RS06100 (1202451)	1202451..1202921	-	471	WP_000824472.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T174430 WP_000052076.1 NZ_CP062342:1198768-1198956 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174430 NZ_CP081008:3063921-3064199 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174430 WP_001059690.1 NZ_CP062342:1198479-1198739 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174430 NZ_CP081008:3063658-3063918 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56