Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2674694..2675208 Replicon chromosome
Accession NZ_CP062340
Organism Staphylococcus aureus strain NAS_AN_275

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag IF742_RS13265 Protein ID WP_000581792.1
Coordinates 2674999..2675208 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag IF742_RS13260 Protein ID WP_000138932.1
Coordinates 2674694..2674987 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF742_RS13240 (2670058) 2670058..2670699 + 642 WP_072473054.1 thiamine phosphate synthase -
IF742_RS13245 (2670785) 2670785..2671657 + 873 WP_000725800.1 membrane protein insertase YidC -
IF742_RS13250 (2672369) 2672369..2673016 - 648 WP_001187630.1 HD domain-containing protein -
IF742_RS13255 (2673044) 2673044..2674528 - 1485 WP_000571549.1 cardiolipin synthase -
IF742_RS13260 (2674694) 2674694..2674987 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
IF742_RS13265 (2674999) 2674999..2675208 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
IF742_RS13270 (2675336) 2675336..2675473 + 138 WP_000828354.1 Lmo0850 family protein -
IF742_RS13275 (2675737) 2675737..2676939 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
IF742_RS13280 (2677257) 2677257..2678327 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
IF742_RS13285 (2678342) 2678342..2679700 + 1359 WP_072473053.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T174422 WP_000581792.1 NZ_CP062340:2674999-2675208 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T174422 NZ_CP081008:2502548-2502898 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT174422 WP_000138932.1 NZ_CP062340:2674694-2674987 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT174422 NZ_CP081008:2502303-2502554 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References