Detailed information of TA system TA174405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2659534..2660063
• Replicon: chromosome
• Accession: NZ_CP062338
• Organism: Staphylococcus aureus strain NAS_NP_103

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF745_RS13085
• Protein ID: WP_000621175.1
• Coordinates: 2659701..2660063 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF745_RS13080
• Protein ID: WP_000948331.1
• Coordinates: 2659534..2659704 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF745_RS13050 (2654570)	2654570..2655130	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF745_RS13055 (2655339)	2655339..2655818	+	480	WP_001287079.1	hypothetical protein	-
IF745_RS13060 (2655811)	2655811..2657394	+	1584	WP_001294620.1	PH domain-containing protein	-
IF745_RS13065 (2657381)	2657381..2657872	+	492	WP_001205912.1	PH domain-containing protein	-
IF745_RS13070 (2657876)	2657876..2658235	+	360	WP_000581197.1	holo-ACP synthase	-
IF745_RS13075 (2658301)	2658301..2659449	+	1149	WP_001281154.1	alanine racemase	-
IF745_RS13080 (2659534)	2659534..2659704	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF745_RS13085 (2659701)	2659701..2660063	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF745_RS13090 (2660413)	2660413..2661414	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF745_RS13095 (2661533)	2661533..2661859	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF745_RS13100 (2661861)	2661861..2662340	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF745_RS13105 (2662315)	2662315..2663085	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174405 WP_000621175.1 NZ_CP062338:2659701-2660063 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174405 NZ_CP081007:c4066749-4066351 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174405 WP_000948331.1 NZ_CP062338:2659534-2659704 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174405 NZ_CP081007:c4067045-4066752 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1