Detailed information of TA system TA174379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1745334..1745848
• Replicon: chromosome
• Accession: NZ_CP062336
• Organism: Staphylococcus aureus strain NAS_NP_161

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: IF748_RS08625
• Protein ID: WP_000581792.1
• Coordinates: 1745334..1745543 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: IF748_RS08630
• Protein ID: WP_000138932.1
• Coordinates: 1745555..1745848 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF748_RS08605 (1740842)	1740842..1742200	-	1359	WP_000611465.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
IF748_RS08610 (1742215)	1742215..1743285	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
IF748_RS08615 (1743603)	1743603..1744805	+	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
IF748_RS08620 (1745069)	1745069..1745206	-	138	WP_000828354.1	Lmo0850 family protein	-
IF748_RS08625 (1745334)	1745334..1745543	-	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
IF748_RS08630 (1745555)	1745555..1745848	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
IF748_RS08635 (1746014)	1746014..1747498	+	1485	WP_000571549.1	cardiolipin synthase	-
IF748_RS08640 (1747526)	1747526..1748173	+	648	WP_001187634.1	HD domain-containing protein	-
IF748_RS08645 (1748900)	1748900..1749772	-	873	WP_000725802.1	membrane protein insertase YidC	-
IF748_RS08650 (1749858)	1749858..1750499	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T174379 WP_000581792.1 NZ_CP062336:c1745543-1745334 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T174379 NZ_CP081000:2824095-2824673 [Mycolicibacterium senegalense]
GTGAAGCGCCTCGATCTCGTCGTCGGCTGCAACGGCGCCGGCAAGTCGACCTTCGTCGAACTCACCCTCTCACCTCTGCT
GCCCGGCAGCCCGTTCGTCAACGCGGACGAAATCGCCAAGCGGCGGTGGCCCGAAGACCCTGCCCCGCATTCGTACGAAG
CCGCGTTGATCGCGGCCGAAACCCGCGCGAAACTCATCGAACTCGGTGAGTCGTTCATCGCCGAAACCGTGTTCTCGCAC
CCTTCCAAGCTCGACCTCATCGACGACGCACACGTCGCCGGGTACACCGTGGTGCTGCACTGCATCCTCATTCCCGAAGA
ATTGGCCGTGCAGCGCGTGCGTCACCGGGTGTCGGCGGGTGGCCACGACGTGCCTGAAGCCAAGATCCGCGAGCGCTACC
AACGGCTTTGGGACCTGGTCGCCGTCGCCGCGATGCGCGCGGACGCCGCAACGTTCTACGACAACAGCACCGTCAGAGGC
CCCCGCATCGTCGCCCAGATAGCGGGCGGGTTCGCCATCGGTTCGCCCACCTGGCCGAAATGGGCACCGGCGGTGCTCAT
TTCACGCTGGGCGGGTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT174379 WP_000138932.1 NZ_CP062336:c1745848-1745555 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT174379 NZ_CP081000:2823668-2824057 [Mycolicibacterium senegalense]
ATGACGGAGACCGCAGACCGGGTCACTCGGTTCGCCGCTGATCTCATCGATAGTGCGGCCGCCGAAGGCGCACGTCAGAG
CCGGTCAGCCAAACAGCAGCTCGACCACTGGGCTCGAGTGGGCCGGGCCGTGTCCAGCCAACACAGTGCGGCACGCCGCA
AGGTGGAGTCCGCGTTGGCCGGCGAGTTGTCCCTGCGTGAGTTGACGGCCGAAGAAGGCACGGTGTTCGACGCCGAGATC
GCCGCCTCCATCGAGGAAAGCCTGGCTCGTGCCGACTACGGCAAGACGCTGGCCGGACGCGGAATCACCACGGTGGCGCT
CGACGACAACGGCAACATCGTGCAGTACCGCCCCGACGGCAGCACTGCGGTGCTGGCACGCACGCAGTGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13