Detailed information of TA system TA174378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1725576..1726105
• Replicon: chromosome
• Accession: NZ_CP062336
• Organism: Staphylococcus aureus strain NAS_NP_161

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF748_RS08535
• Protein ID: WP_000621175.1
• Coordinates: 1725576..1725938 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF748_RS08540
• Protein ID: WP_000948331.1
• Coordinates: 1725935..1726105 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF748_RS08515 (1722555)	1722555..1723325	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
IF748_RS08520 (1723300)	1723300..1723779	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF748_RS08525 (1723781)	1723781..1724107	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF748_RS08530 (1724226)	1724226..1725227	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF748_RS08535 (1725576)	1725576..1725938	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF748_RS08540 (1725935)	1725935..1726105	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF748_RS08545 (1726190)	1726190..1727338	-	1149	WP_000130547.1	alanine racemase	-
IF748_RS08550 (1727404)	1727404..1727763	-	360	WP_000581202.1	holo-ACP synthase	-
IF748_RS08555 (1727767)	1727767..1728258	-	492	WP_001205912.1	PH domain-containing protein	-
IF748_RS08560 (1728245)	1728245..1729828	-	1584	WP_001294620.1	PH domain-containing protein	-
IF748_RS08565 (1729821)	1729821..1730300	-	480	WP_001287079.1	hypothetical protein	-
IF748_RS08570 (1730509)	1730509..1731069	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174378 WP_000621175.1 NZ_CP062336:c1725938-1725576 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174378 NZ_CP080999:5260918-5261316 [Mycobacterium malmoense]
ATGATCGTCGACACGTCGGTCTGGATTGAATTTCTGACCACCTCTGAGTCGCTCGCCAGCCGCTGGCTAGCAGATCGCAT
TGCCGCCAACTCGCAGGTGATCGTCCCTGAGGTCGTGATGATGGAACTGCTGATCGGTACGACCGACGAGTCCAAGGCAA
TACGGCGGAGGCGACTACTGCAACGGTTCAATATCGAGCCGTTTGCCCCCGTGCGGGACGCAGAAGATGCGGCCGCTATC
CACCGGCGCTGTCGTCGCGGTGGTGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGCTGCGGATCGG
GGTCACTGTGGCACATCGTGATCGTGATTACGAGGTGATCCGCACGCATTGCGGACTACAAACCGAGCCCTTGTTCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174378 WP_000948331.1 NZ_CP062336:c1726105-1725935 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174378 NZ_CP080999:5260690-5260917 [Mycobacterium malmoense]
ATGTCGCGGACCAACATCGAAATCAACGACGAACTTGTGGCCGCCGCGCAGCGAATGTATCGACTGGATTCGAAGCGGAG
TGCCGTTGACCTCGCACTGCGCAGGCTCGTGGGTGAACCGTTAGACCGCGATGAGGCGTTGGCACTCCAGGGCAGCGGTT
TCGATTTCACCAATGATGAGATCGAATCGTTTTCGGACACGGGCATAGACCTCGCAGACCAGTCTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1